Stage top chamber

Manufactured by Okolab

Sourced in Italy

The Stage Top Chamber is a compact and versatile environmental control system designed to maintain precise temperature, gas concentration, and humidity within a defined sample area on a microscope stage. It provides a controlled microenvironment for live-cell imaging and other sensitive applications.

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Lab products found in correlation

Lunv solid state laser launch, by Nikon (2 mentions) Elements ar 4, by Nikon (2 mentions) Csu w1, by Hamamatsu Photonics (2 mentions) Glass bottom dishes, by MatTek (2 mentions) Eclipse ti, by Yokogawa (1 mentions) Plan apo, by Nikon (1 mentions) Flash 4, by Hamamatsu Photonics (1 mentions) Csu x1 spinning disk confocal, by Yokogawa (1 mentions) Inverted tie microscope, by Nikon (1 mentions) Lsm 700, by Zeiss (1 mentions) Eclipse ti inverted confocal microscope, by Nikon (1 mentions) Orca flash 4.0 cmos camera, by Hamamatsu Photonics (1 mentions) Ultraview vox, by PerkinElmer (1 mentions) Volocity software, by Quorum Technologies (1 mentions) N a 1.49 apo tirf objective lens, by Nikon (1 mentions) Orca flash 4.0 scmos camera, by Hamamatsu Photonics (1 mentions) Eclipse ti inverted microscope, by Nikon (1 mentions)

Spelling variants of this product

Stage-top incubation chamber (4 citations) Uno-combined controller stage top incubation chamber (3 citations) Microscope stage top incubation chamber (3 citations)

5 protocols using stage top chamber

Imaging of Neomycin-Induced Hair Cell Damage

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Images were acquired using a Nikon Ti Eclipse with Yokogawa CSU-W1 spinning disk head equipped with a Hamamatsu Flash 4.0 sCMOS. Objective lenses used were a Nikon Plan Apo 40 × 1.15 NA LWD (water) and a Nikon Plan Apo 20 × 0.75 NA.
For live imaging experiments, larvae were immobilized with tricaine (MS-222) up to 150 mg/L and mounted in glass bottom dishes (MatTek) with 0.8% low melting point agarose dissolved in 0.5x E2 with tricaine (100 mg/L). Time lapse recordings were started 10-min after addition of neomycin (300μM) on top of the agarose. Temperature was kept constant at 28.5 °C using a Stage Top Chamber (OkoLab).
A Nikon LUNV solid state laser launch was used for lasers 405, 445, 488, 561, and 647 nm. Emission filters used on the Nikon were 480/30, 535/30, 605/70.
All image acquisition was performed using Nikon Elements AR 4.6 (Nikon) software.

Denans N., Tran N.T., Swall M.E., Diaz D.C., Blanck J, & Piotrowski T. (2022). An anti-inflammatory activation sequence governs macrophage transcriptional dynamics during tissue injury in zebrafish. Nature Communications, 13, 5356.

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Imaging Mitotic and Interphase eRPE1 Cells

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eRPE1 cells were grown on 35-mm glass bottom μ-dishes (ibidi, Cat#50-305-807). Cells were imaged in culturing media; 37°C and 5% CO₂ was maintained using a cage incubator and a stage top chamber (OkoLab). Time-lapse z-stack images were captured on an inverted Ti-E microscope (Nikon) equipped with a CSU-X1 spinning disk confocal (Yokogawa), motorized XY stage with Z piezo (ASI), 4 line laser launch (Vortan), Lambda 10–3 emission filter wheel (Sutter), quad-band dichroic ZET 405/488/561/640x (Chroma), with Plan Apo VC 100x/1.4NA and Plan Apo VC 60x/1.3NA oil objectives, and a Photometrics Prime95B sCMOS camera (Teledyne). eRPE1 cells were imaged using a 488-nm laser and ET525/50m emission filter (Chroma). z-stacks were acquired every 20–30 sec for 1 hr for mitotic cells (Figs. 2, 3, 5) using the 100x/1.4NA objective, and every 15–30 min for up to 10 hours for interphase cells (Fig. 4) using the 60x/1.3NA objective. Image processing was conducted using Imaris software.

Cai P., Casas C.J., Plancarte G.Q., Mikawa T, & Hua L.L. (2024). Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle. Research Square.

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Confocal Imaging of Zebrafish Development

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Images were acquired using the confocal microscopes Zeiss LSM700, Zeiss LSM780 or Nikon Ti Eclipse with Yokogawa CSU-W1 spinning disk head equipped with a Hamamatsu Flash 4.0 sCMOS. Objective lenses used were Zeiss Plan-Apochromat 10x/0.45 M27 (air), Plan-Apochromat 20x/0.8 M27 (air) and LD C-Apochromat 40x/1.1 W Korr M27 (water) for the Zeiss Microscopes and a Nikon Plan Apo 40× 1.15NA LWD (water). Temperature was kept constant at 28.5°C using Zeiss 780 standard incubation or a Stage Top Chamber (OkoLab) for the Nikon Microscope.
For live imaging experiments, larvae were immobilized with tricaine (MS-222) up to 150 mg/L and mounted in glass bottom dishes (MatTek) with 0.8% low melting point agarose dissolved in 0.5x E2 or 5x E2 with tricaine (100 mg/L). Time lapse recordings during Notch inhibition were performed after a 30-minute pre-incubation period with LY411575 (50uM). Larvae were exposed to the same concentration throughout the experiment.
Laser lines used on the Zeiss confocal were Diode 405–30, Argon multiline laser (458, 488 and 514 nm), DPSS 561–10, HeNe 594 and 633 nm. A Nikon LUNV solid state laser launch was used for lasers 445, 515, and 561nm for CFP, YFP, and RFP respectively. Emission filters used on the Nikon were 480/30, 535/30, 605/70.
All image acquisition was performed using Zen 2012 SP5 Black (Zeiss) and Nikon Elements AR 4.6 (Nikon) software.

Peloggia J., Münch D., Meneses-Giles P., Romero-Carvajal A., Lush M.E., Lawson N.D., McClain M., Pan Y.A, & Piotrowski T. (2021). Adaptive cell invasion maintains lateral line organ homeostasis in response to environmental changes. Developmental cell, 56(9), 1296-1312.e7.

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Time-lapse and Confocal Microscopy of E. coli

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Electron microscopy was performed as previously described (Noland et al., 2017) (link). For time-lapse microscopy, WT CFT073, CFT073Dlgt and CFT073DlgtDlpp cells were grown overnight in LB medium containing 4% arabinose, back-diluted to a final OD600 of 0.1 and immediately placed between a cover slip and 1% agarose pad containing 0.2% glucose for imaging. Cells were maintained at 37 °C during imaging in a stage top chamber (Okolab Inc.). Cells were imaged on a Nikon Eclipse Ti inverted confocal microscope (Nikon Instruments Inc.) coupled with a UltraVIEW VoX (PerkinElmer Inc.) and a 100× (NA 1.40) oil-immersion objective. Images were captured at various times using ORCA-Flash 4.0 CMOS camera (Hamamatsu Photonics), collected using Volocity software (Quorum Technologies) and processed using Fiji (Schindelin et al., 2012) (link). For confocal microscopy, images were acquired on a Leica SP8 STED 3x platform using a 100× white light, NA:1.4 oil immersion objective. CFT073imp4213 cells were treated with Lgti, LspAi or LolCDEi at 1×MIC for 30 minutes, fixed with 4% paraformaldehyde and incubated with 1 µg/mL FM-64 dye and 1 µg/mL DAPI solution. Quantitation of bacterial cell area was performed using the ImageJ program by measuring at least ~100 bacterial cells from two independent experiments.

Diao J., Komura R., Sano T., Pantua H., Storek K.M., Inaba H., Ogawa H., Noland C.L., Peng Y., Gloor S.L., Yan D., Kang J., Katakam A.K., Nickerson N.N., Austin C.D., Murray J., Rutherford S.T., Reichelt M., Xu Y., Xu M., Yanagida H., Nishikawa J., Reid P., Cunningham C.N., & Kapadia S.B. (2020). Novel inhibitors ofE. colilipoprotein diacylglyceryl transferase are insensitive to resistance caused bylppdeletion.

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Live-cell TIRF Microscopy Imaging

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Live-cell Total Internal Reflection Fluorescence (TIRF) Microscopy TIRF imaging of live cells were performed using a Nikon Eclipse Ti inverted microscope (Nikon Instruments), equipped with a motorized TIRF illuminator, with a polarization-maintaining optical fiber-coupled laser combiner (100 mW 405 nm, 60 mW 488nm, 50 mW 561 nm, and 100 mW 642nm solid-state lasers, Omicron Laserage), a light emitting diode-based epifluorescence excitation source(-SOLA, Lumencor), an ORCA-flash 4.0 sCMOS camera (Hamamatsu), a 60X N.A 1.49 Apo TIRF objective lens (Nikon Instruments), and an Okolab stage-top chamber with CO 2 and temperature control (Okolab, Italy).

Xia S., Lim Y.B., Zhang Z., Wang Y., Zhang S., Lim C.T., Yim E.K.F, & Kanchanawong P. (2019). Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells. Cell reports, 28(5).

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