Sac val

Complete Freund’s adjuvant (CFA) was bought from Sigma (Shanghai, China). Sac/Val and valsartan were purchased from Novartis Pharma AG (Basel, Switzerland). Antibodies against IFN-γ (Cat# sc-8423, RRID: AB_627,778), IL-1β, IL-17 (Cat# sc-374218, RRID: AB_10988239), and β-actin were bought from Santa Cruz (Dallas, USA). Anti-IL-18 antibody was purchased from Arigobio (Hsinchu City, Taiwan). The IL-6 ELISA kit (Cat# M6000B, RRID: AB_2877063) and the hsCRP ELISA kit were purchased from R andD (Minneapolis, USA). The IFN-γ and IL-17 ELISA kits were purchased from Neobioscience (Shenzhen, China). The cTnT quantitative rapid assay kit and collagenase B were bought from Roche Diagnostics (Shanghai, China). The total protein extraction kit, super ECL reagent, and antibody against ROR-γt (Cat# 14-6988-80, RRID: AB_1311291) were bought from Thermo Fisher Scientific Inc. (Hanover Park, USA). Antibodies against NLRP3 (Cat# AG-20B-0014, RRID: AB_2490202), ASC (Cat# AG-25B-0006, RRID: AB_2490440) and caspase-1 (Cat# AG-20B-0042, RRID: AB_2490248) were purchased from AdipoGen. Antibodies against T-bet, sGC-α1, sGC-β1, total-NF-κB p65 and phospho-NF-κB p65 were purchased from ImmunoWay (Plano, United States). FITC-anti-mouse CD4, PE-anti-mouse IFN-γ and allophycocyanin-anti-mouse IL-17 were purchased from eBioscience.