Cell surface and intracellular staining was performed as previously described (15 (link), 47 (link)). Antibodies used: anti-CD3 Bv650 (OKT3, Biolegend), anti-CD3 FITC (SK7, BD Biosciences), anti-Vα7.2 PE (3C10, Biolegend), anti-CD161 Pe-Cy5 (DX12, BD Biosciences), anti-CD4 Bv711 (OKT4, Biolegend), anti-CD8 Bv570 (RPA-T8, Biolegend), anti-CD69 BUV737 (FN50, BD Biosciences), anti-CD107a BUV395 (H4A3, BD Biosciences), anti-GzB FITC (GB11, Biolegend), anti-IFNγ APC (25723.11, BD Biosciences), anti-TNF PE-Cy7 (Mab11, BD Biosciences), anti-IL17A Bv421 (BL168, Biolegend), anti-RORγt APC (R&D system), anti-RORγt Bv650 (Q21–559, BD Biosciences), anti-PLZF PECF594 (R17–809, BD Biosciences), anti-T-bet Bv711 or Bv605 (4B10, Biolegend), anti-MR1 (26.5, Biolegend), anti-HLA-DR (L243, Biolegend), anti-HLA-A2 (BB7.2, Biorad), LIVE/DEAD Fixable Aqua and Near-IR dye (Invitrogen). Flow cytometry data was acquired on BD LSRFortessa or BD Symphony A5 instruments (both BD Biosciences) and analyzed using FlowJo software v. 10.5.3 (TreeStar). Analyses of MAIT cell polyfunctionality were performed using the SPICE software v. 6.0 (48 (link)).
Anti rorγt apc
Manufactured by BD
Sourced in United States
The Anti-RORγt APC is a laboratory reagent used in flow cytometry applications. It is a fluorescently-labeled antibody that specifically binds to the nuclear receptor RORγt, which is a key transcription factor involved in the differentiation and function of T helper 17 (Th17) cells. This reagent can be used to identify and quantify Th17 cells in various biological samples.
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Lab products found in correlation
Anti ifn γ apc, by BD (1 mentions)
Anti cd3 fitc sk7, by BD (1 mentions)
Anti hla dr, by BioLegend (1 mentions)
Symphony a5, by BD (1 mentions)
Anti il 17a bv421, by BioLegend (1 mentions)
Anti mr1 26, by BioLegend (1 mentions)
Anti cd4 bv711 okt4, by BioLegend (1 mentions)
Anti cd107a buv395 h4a3, by BD (1 mentions)
Anti rorγt bv650, by BD (1 mentions)
Anti plzf pe cf594, by BD (1 mentions)
Lsrfortessa, by BD (1 mentions)
Anti il 4 pe, by BD (1 mentions)
Anti ifn γ pe, by BD (1 mentions)
Anti t bet apc, by BD (1 mentions)
Anti foxp3 apc, by BD (1 mentions)
Anti il 17a apc, by BD (1 mentions)
Anti ctla 4 apc, by BD (1 mentions)
Facsuite software, by BD (1 mentions)
Facsverse, by BD (1 mentions)
Golgiplug, by BD (1 mentions)
2 protocols using anti rorγt apc
1
Multiparametric Flow Cytometry of MAIT Cells
2
Phenotypic Analysis of T-cell Responses to Mycobacterial Infection
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PBMCs (2 × 105 cells) were stimulated with M. avium bacilli and M. intracellulare bacilli individually for 48 h and incubated with GolgiPlug (1 μL/mL; BD Pharmingen, San Diego, CA, USA) for the final 5 h of culture. Cells were measured by flow cytometry using a fluorescence activated cell sorter (FACS) (FACSVerse, BD Biosciences, San Jose, CA, USA) and anti-CD3-APC-Cy7, anti-CD4-FITC, anti-IFN-γ-PE, anti-IL-4-PE, anti-IL-17A-APC, anti-CD25-PE, anti-Foxp3-APC, anti-T-bet-APC, anti-GATA3-APC, and anti-RORγT-APC antibodies (BD Pharmingen, San Diego, CA, USA). The PD-1, CTLA-4, and TIM-3 were also stained with anti-PD-1-PE, anti-CTLA-4-APC, and anti-TIM-3- APC (BD Biosciences, San Diego, CA, USA). Data were analyzed using BD FACSuite software (BD Biosciences, San Jose, CA, USA). A gate was set on the lymphocytes using characteristic forward scatter and side scatter parameters followed by CD3+CD4+ T cells (Supplementary Figure S1 ).
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