Hematoxylin–eosin (HE) and immunohistochemical staining were performed by standard techniques as previously described. The following primary antibodies were used: anti-CD34 (ab81289; Abcam), anti-NSE (PA5-27,452; Invitrogen), anti-D2-40 (MA1-83,884; Invitrogen), anti-CA9 (ab184006; Abcam), anti-PCK (ab28455; Abcam), anti-Oligo2 (ab109186; Abcam), anti-SOX9 (#82,630; Cell signaling technology), anti-Inhibin (PA5-81,202; Invitrogen), anti-GFAP (ab7260; Abcam), anti-S100 (ab183979; Abcam), and anti-Ki67 (ab15580; Abcam).
Ab184006
Manufactured by Abcam
Sourced in United Kingdom
Ab184006 is a laboratory product manufactured by Abcam. It is a scientific instrument designed for use in research and experimental settings. The core function of this product is to [description not available].
Automatically generated - may contain errors
Lab products found in correlation
Ab183979, by Abcam (1 mentions)
Ab109186, by Abcam (1 mentions)
Ab28455, by Abcam (1 mentions)
Ab7260, by Abcam (1 mentions)
Ab81289, by Abcam (1 mentions)
Ab15580, by Abcam (1 mentions)
C6198, by Merck Group (1 mentions)
Bromodeoxyuridine (brdu), by Biosynth (1 mentions)
4.3.11.3 mouse mab, by Hypoxyprobe (1 mentions)
Mca497ga, by Bio-Rad (1 mentions)
Af1320, by R&D Systems (1 mentions)
Ab125212, by Abcam (1 mentions)
Ab182422, by Abcam (1 mentions)
Ab28364, by Abcam (1 mentions)
4 protocols using ab184006
1
Immunohistochemical Staining Protocol
2
Histological Analysis of Mouse Uterus
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Five micrometer mouse uterine sections were stained with haematoxylin and eosin (H&E) and stage of breakdown/repair graded by two masked independent observers using a previously published scoring system41 (link) (Supplementary Fig. 2 ). Mouse uterine sections were also stained for endoglin (1:50, R&D Systems, AF1320, Abingdon, UK), alpha smooth muscle actin (αSMA, 1:250, C6198, Sigma, Dorset, UK), Ly6G (BioLegend, 127601, 1:1000. London, UK), F4/80 (Bio-Rad, MCA497GA, 1:50. Oxford, UK) and carbonic anhydrase IX (Abcam, ab184006, 1:2000, Cambridge, UK). Proliferating cells were identified using anti-bromodeoxyuridine antibody (BrdU, 1:1500, Fitzgerald, Acton, MA, USA) and hypoxia detected using anti-pimonidazole antibody as per manufacturer’s instructions (4.3.11.3 mouse MAb, Hypoxyprobe, Burlington, USA).
3
Immunohistochemical Detection of CA9
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4
Tumor Tissue Preservation and Staining
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Tumors were placed in a 50 mL conical centrifuge tube of 10% neutral buffered formalin for 24 h at 4 °C. After 24 h, formalin was replaced with 50 mL phosphate-buffered saline (PBS), then placed in 50 mL 70% ethanol before shipment (IHC World, Ellicott City, MD, USA) for IHC staining. Tumors were paraffin-embedded, sectioned in 5 µm sections, and stained for anti-CD68 (Abcam®, ab125212), anti-CD163 (Abcam®, ab182422), anti-carbonic anhydrase-9 (CA-IX) (Abcam®, ab184006), anti-CD31 (Abcam®, ab28364), and H&E.
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