The following antibodies for flow cytometry were used: anti-CD107a-APC (H4A3), anti-CD3-AF700 (UCHT1), anti-CD3-PE-CF594 (UCHT1), anti-CD56-PE-Cy7 (B159), anti-CD56-PerCP Cy5.5 (B159), anti-CD57-PE (NK-1), anti-CD45-PE-Cy5 (HI30), anti-NKG2D-BV605 (1D11), anti-NKG2D-PE-CF594 (1D11), anti-CD16-BV510 (3G8), anti-DNAM-1-BV786 (DX11), anti-PD-1-BV421 (MIH4), anti-IFNγ-BV650 (4S.B3), anti-CD14-BV605 (M5E2), anti-CD19-BV650 (SJ25C1) purchased from BD Biosciences; anti-DNAM-1-APC (11A8), anti-NKp46-PE-Cy7 (9E2), anti-TNFα-AF700 (Mab11), anti-CD96-APC (NK92.39) purchased from Biolegend; anti-NKp30-PE (Z25), anti-KIR2DL1/2DS1-PE Cy5.5 (EB6B), anti-KIR2DL2/L3/S2-PE (GL-183) purchased from Beckman Coulter; anti-NKG2A-AF700 (131411), anti-KIR3DL1-APC (DX9) purchased from R&D Systems; anti-NKG2A-FITC (REA110), anti-NKG2C-PE (REA205) purchased from Miltenyi; anti-TIGIT-APC (MBSA43) purchased from eBioscience. All these antibodies were used according to the manufacturers’ protocol. Prior to surface staining, NK cells were pre-stained with Live/Dead™ Fixable Near-IR Dead Cell Stain Kit (Invitrogen). Flow cytometry was performed by using FACSCantoTM II (BD Biosciences) or Cytoflex (Beckman Coulter) and analyzed by FlowJo Software.
Anti cd16 bv510
Manufactured by BD
Sourced in Germany
Anti-CD16-BV510 is a fluorescently-labeled monoclonal antibody that binds to the CD16 surface antigen. CD16 is a low-affinity Fc receptor that is expressed on natural killer cells, neutrophils, and some monocytes. The BV510 fluorophore allows for detection and analysis of CD16-positive cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd107a apc, by BD (1 mentions)
Anti cd56 percp cy5, by BD (1 mentions)
Anti cd3 af700, by BD (1 mentions)
Anti cd57 pe, by BD (1 mentions)
Anti nkg2d pe cf594, by BD (1 mentions)
Anti cd3 pe cf594, by BD (1 mentions)
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Facscanto 2, by BD (1 mentions)
Anti cd56 pe cy7, by BD (1 mentions)
Anti cd94 pe, by BD (1 mentions)
Anti cd45 apc h7, by BD (1 mentions)
Anti cd335, by BD (1 mentions)
Facsdiva software v7, by BD (1 mentions)
7 aad, by BD (1 mentions)
Facsaria 2, by BD (1 mentions)
Anti cd56 apc, by BD (1 mentions)
Anti cd3 percp cy5, by BD (1 mentions)
2 protocols using anti cd16 bv510
1
NK Cell Phenotyping by Flow Cytometry
2
Comprehensive Immunophenotyping of NK Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For each patient, cryopreserved PBMCs from week 0 to week Pt-24 were thawed and tested. PBMCs of healthy donors were included in this experiment. Thawed PBMCs were stained with anti-CD45-APC-H7, anti-CD3-PerCP-Cy5.5, anti-CD56-APC, anti-CD16-BV510, anti-CD94-PE, anti-CD335 (NKp46) -PE-Cy7, anti-CD336 (NKp44) -BB515, anti-CD337 (NKp30) - BV421 (BD Bioscience), anti-CD314 (NKG2D)-PE-Cy7 (Biolegend), anti-CD159a (NKG2A)-PE (R and D Systems) and anti-CD159C (NKG2C)-VioBright™FITC (Miltenyi Biotech, Bergisch Gladbach, Germany) for 15 min and with 7-AAD (BD Bioscience) for 10 min before being detected by flow cytometry (BD FACSAria II, BD Bioscience). Data were analyzed with BD FACSDiva Software v7.0.
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