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Microplate manager software v6

Manufactured by Bio-Rad
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Sourced in United States

Microplate Manager® Software v6.3 is a comprehensive software solution for the management and analysis of microplate-based assays. It provides users with a suite of tools to streamline data acquisition, processing, and reporting for a wide range of laboratory applications.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Halt protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions)

Spelling variants of this product

Microplate Manager software (20 citations) Microplate Manager (15 citations) Microplate Manager v6.0 (5 citations)

2 protocols using microplate manager software v6

1

Antioxidant Capacity Evaluation

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The antioxidant capacity of the end-products was estimated using the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and the 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assay, which are among the most used methods to estimate antioxidant capacity methods [34 (link)]. Assays were performed in 96-well plates in triplicate. Briefly, absorbance measurements were performed using an iMarkTM microplate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA), with Microplate Manager® Software v6.3 (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
Sánchez-Suárez J., Villamil L., Coy-Barrera E, & Díaz L. (2021). Cliona varians-Derived Actinomycetes as Bioresources of  Photoprotection-Related Bioactive End-Products. Marine Drugs, 19(12), 674.
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2

Quantification of Lung Cytokines

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After euthanasia, the trachea was cannulated and lungs were lavaged with HBSS (2 × 0.5 ml). Lungs were then collected. BALF and lungs were stored at −80°C for cytokine assays. Lungs were homogenized with a glass dounce in 1.0 ml PBS with Halt protease and phosphatase inhibitor (Thermo Scientific). The homogenates were centrifuged at 10,000 g at 4°C for 15 min, and protein concentrations in the supernatant were quantified with BCA Protein Assay kit (Thermo Scientific). Levels of IL-5, IL-13, and IL-33 in lung homogenate and BAL supernatants were measured by Quantikine ELISA kits (R&D systems) per manufacturer instructions. Data were collected using Microplate Manager Software v6.3 (Bio-Rad Laboratories).
Block K.E., Iijima K., Pierson M.J., Walsh D.A., Tei R., Kucaba T.A., Xu J., Khan M.H., Staley C., Griffith T.S., McSorley H.J., Kita H, & Jameson S.C. (2022). Physiological microbial exposure transiently inhibits mouse lung ILC2 responses to allergens. Nature immunology, 23(12), 1703-1713.
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