Mrs1754

All animal experiments were conducted in accordance with the guidelines of Committee for the Purpose of Control and Supervision of Experiment on Animals, India. Male C57BL/6 mice (body weight: 22 ± 2 g) were a kind gift from Zydus Research Centre, Ahmedabad. Animals were maintained at room temperature in a light (12 h light/12 h dark)-controlled environment with access to food and water ad libitum. One week after the acclimatization animals were divided into six different treatment groups (with two main subgroups: Control and CsA treated) as depicted in Table 1. A total of three groups (CsA treated) of animals were subjected to and maintained on low-salt diet throughout the trial. The salt depletion has been reported to enhance sensitivity to CsA-induced nephrotoxicity. After 1 week on a low-salt diet, the mice were injected daily with 25 mg/kg/day CsA subcutaneously for 5 weeks. The olive oil was used as vehicle for CsA; hence, in the vehicle control group, animals were injected equivalent amount of olive oil. Following 5 weeks, the A_2B AR modulators were administered intraperitoneally for 2 weeks. The dose of MRS1754 (Abcam plc. UK) was 1 mg/kg. The dose of NECA (Abcam plc.UK) was 50 μg/kg of body weight. The control groups of animals were also treated with A_2B AR modulators.