Anti synaptophysin rabbit polyclonal

Manufactured by Abcam

Sourced in United Kingdom

Anti-synaptophysin rabbit polyclonal is a primary antibody that recognizes the synaptophysin protein. Synaptophysin is a integral membrane protein found in presynaptic vesicles and is commonly used as a marker for synaptic density and neurotransmitter release.

Automatically generated - may contain errors

Lab products found in correlation

Anti blbp, by Abcam (1 mentions) Anti aldh1l1, by Merck Group (1 mentions) Anti eaat 2, by Abcam (1 mentions) Anti iba1, by Merck Group (1 mentions) Anti vglut1, by Merck Group (1 mentions) Anti gfap, by Agilent Technologies (1 mentions) Anti aβ42 rabbit polyclonal, by Abcam (1 mentions) Anti acetylated α tubulin clone 6 11b 1, by Merck Group (1 mentions) Pierce bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Enhanced chemiluminescence reagent, by GE Healthcare (1 mentions) Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions) Superoxide dismutase sod assay kit, by Cayman Chemical (1 mentions) Malondialdehyde mda assay kit, by Cayman Chemical (1 mentions) Protease inhibitor, by Merck Group (1 mentions) Donepezil, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Plan neofluar, by Zeiss (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Lsm 3.0 image analysis software, by Zeiss (1 mentions) Axiovert 200, by Zeiss (1 mentions)

Spelling variants of this product

Synaptophysin (74 citations) Anti-synaptophysin (38 citations) Rabbit anti-synaptophysin (15 citations) Rabbit anti- (5 citations)

4 protocols using anti synaptophysin rabbit polyclonal

Immunohistochemical Profiling of Alzheimer's Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used for this study: anti‐oligomeric Aβ OC rabbit polyclonal (1:5000, Millipore, Burlington, MA); anti‐GFAP (glial fibrillary acidic protein) rabbit polyclonal (1:10000, Dako, Troy, MI); anti‐GFAP chicken polyclonal (1:20000, Millipore); anti‐BLBP (brain lipid‐binding protein) rabbit polyclonal (1:2000, Abcam, Cambridge, MA); anti‐AQP4 (aquaporin 4) rabbit‐polyclonal (1:10000, Sigma, St. Louis, MO); anti‐ALDH1L1 (aldehyde dehydrogenase 1 family member L1; N103/39 clone) mouse monoclonal (1:2000, Millipore); anti‐EAAT2 (excitatory amino acid transporter 2) rabbit polyclonal (1:10000, Abcam); anti‐human APP rabbit polyclonal (1:10000, Sigma); anti‐Iba1 (ionized calcium binding adaptor molecule 1) rabbit polyclonal (1:1000, Wako, Mountain View, CA); anti‐VGluT1 (vesicular glutamate transporter 1) guinea‐pig polyclonal (1:10000, Millipore); anti‐synaptophysin rabbit polyclonal (1:1000, Abcam).

Gomez‐Arboledas A., Davila J.C., Sanchez‐Mejias E., Navarro V., Nuñez‐Diaz C., Sanchez‐Varo R., Sanchez‐Mico M.V., Trujillo‐Estrada L., Fernandez‐Valenzuela J.J., Vizuete M., Comella J.X., Galea E., Vitorica J, & Gutierrez A. (2017). Phagocytic clearance of presynaptic dystrophies by reactive astrocytes in Alzheimer's disease. Glia, 66(3), 637-653.

+ Open protocol

+ Expand

Antibody Panel for Alzheimer's Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies and dilutions were used: anti-Aβ42 rabbit polyclonal (1:5,000, Abcam); anti-Aβ (clone 6E10) mouse monoclonal (1:5,000, Signet); anti-human Aβ, N-terminus (clone 82E1) mouse monoclonal (1:6,000, IBL); anti-acetylated α-tubulin (clone 6-11B-1) mouse monoclonal (1:50,000, Sigma); anti-human amyloid precursor protein (hAPP) rabbit polyclonal (1:20,000, Sigma); anti-ATP synthase β (clone 10/ATP) mouse monoclonal (1:10,000, Brand BD Transduction Laboratories); anti-β-Actin (clone AC 74) mouse monoclonal (1:10,000, Sigma-Aldrich); anti-phospho-PHF-tau pSer202/Thr205 (clone AT8) mouse monoclonal (IHC 1:1,000, Cell Signaling); anti-PSD95 rabbit monoclonal (1:2,000, Cell signaling); anti-Somatostatin (SOM) goat polyclonal (1:1,000, Santa Cruz Biotechnology); anti-synaptophysin rabbit polyclonal (1:1,000, Abcam); anti-total tau (TAU46) mouse monoclonal (1:1,000, Cell Signaling) and anti-ubiquitin rabbit polyclonal (1:5,000, Dako).

Fernandez-Valenzuela J.J., Sanchez-Varo R., Muñoz-Castro C., De Castro V., Sanchez-Mejias E., Navarro V., Jimenez S., Nuñez-Diaz C., Gomez-Arboledas A., Moreno-Gonzalez I., Vizuete M., Davila J.C., Vitorica J, & Gutierrez A. (2020). Enhancing microtubule stabilization rescues cognitive deficits and ameliorates pathological phenotype in an amyloidogenic Alzheimer’s disease model. Scientific Reports, 10, 14776.

+ Open protocol

+ Expand

Theracurmin Neuroprotective Effects Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Theracurmin was donated by Handok Co (Seoul, Korea). Theracurmin contains curcumin (30%) as an active compound within a vegitable gum (70%) as submicron form. Donepezil and protease inhibitor were purchased from Sigma Aldrich (St. Louise, MO, USA). Enhanced chemiluminescence reagent was purchased from GE Healthcare Life Sciences (Chicago, IL, USA). Mouse monoclonal anti-post synaptic protein (PSD) 95 and anti-β actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-synaptophysin and goat polyclonal anti-ionized calcium binding adaptor molecule 1 (Iba-1) antibodies were purchased from Abcam (Cambridge, UK). Vectorstain avidin biotinyled enzyme complex (ABC) kit were purchased from Vector Lab (Burlingame, CA, USA). A pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Fisher (Waltham, MA, USA). Superoxide dismutase (SOD) assay kit and malondialdehyde (MDA) assay kit were purchased from Cayman Chemical (Ann Arbor, MI, USA). A glutathione (GSH) reaction kit was purchased from BioVision (Mountainview, CA, USA). All of the chemicals were of highest grade commercially available.

Kim J., Kim J., Huang Z., Goo N., Bae H.J., Jeong Y., Park H.J., Cai M., Cho K., Jung S.Y., Bae S.K, & Ryu J.H. (2019). Theracurmin Ameliorates Cognitive Dysfunctions in 5XFAD Mice by Improving Synaptic Function and Mitigating Oxidative Stress. Biomolecules & Therapeutics, 27(3), 327-335.

+ Open protocol

+ Expand

Immunofluorescence Labeling of Neuromuscular Junctions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The diaphragms and EDL muscles were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, incubated in blocking buffer (10% goat serum in phosphate-buffered saline) and stained with the primary antibody (rabbit polyclonal anti-synaptophysin; 1:500 dilution; Abcam, Cambridge, UK; HPA-GAP43 rabbit polyclonal anti-GAP43; 1:500 dilution; Sigma-Aldrich; or mouse monoclonal anti-CaM; 1:50 dilution; Merck Millipore, Darmstadt, Germany). The primary antibodies were revealed using the specific secondary antibody (goat anti-rabbit or anti-mouse Alexa Fluor-488, or goat anti-rabbit Alexa Fluor-568; 1:200 dilution, Life Technologies, Monza, Italy). The diaphragms were then incubated with 2 μg/ml α-bungarotoxin tetramethylrhodamine conjugate (Life Technogies), for 30 min at room temperature.
Images were acquired using a Zeiss system (LSM 510 META; Jena, Germany) equipped with an inverted microscope (Zeiss Axiovert 200) and an oil-immersion objective (Plan Neofluar; 40 × /1.3 NA, 100 × /1.3 NA). Quantitative analyses on the acquired images were obtained using the Zeiss LSM software version 3.0. The data for the fluorescence image profiles were obtained using the LSM 3.0 image analysis software (Zeiss).

Caprara G.A., Morabito C., Perni S., Navarra R., Guarnieri S, & Mariggiò M.A. (2016). Evidence for Altered Ca2+ Handling in Growth Associated Protein 43-Knockout Skeletal Muscle. Frontiers in Physiology, 7, 493.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!