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Ly6c g or gr 1 rb6 8c5 percpcy5

Manufactured by Thermo Fisher Scientific

Ly6C/G or Gr-1 (RB6–8C5)-PercPCy5.5 is a fluorescence-conjugated antibody that can be used for the identification and analysis of Ly6C and Ly6G-positive cells in flow cytometry applications. This antibody is specific for the Gr-1 antigen, which is expressed on myeloid cells, including granulocytes and monocytes.

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2 protocols using ly6c g or gr 1 rb6 8c5 percpcy5

1

Comprehensive Immune Phenotyping Protocol

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TCR-β (H57–597), F4/80 (BM8), CD2 (RM2–5), CD3e (145–2C11), CD4 (GK1.5), CD8b (53–6.7), CD19 (eBio1D3), CD45R (B220, RA3–6B2), Gr-1 (Ly6G, RB6–8C5), Cd11b (Mac1, M1/70), Ter119 (Ly76) and NK1.1 (Ly53, PK136)-FITC were all from eBioscience and used for lineage determination. c-Kit (CD117, ACK2)-APCeFluor780 from eBioscience, Sca-1-Pacific blue from Biolegend, FcgRII/lll-PE (CD 16/32, 2.4G2), CD34 (RAM34)-AlexaFluor 647, CD135 (Flt3, A2F10)-PE, CD150 (Slamfl, TC15–12F12.2)-PECy7 were from Biolegend and used to quantify HSPCs and progenitor subsets. Peripheral leukocytes were stained with CD115 (AFS98)-APC, CD45 (30-F11)-APCCy7 and Ly6C/G or Gr-1 (RB6–8C5)-PercPCy5.5 from eBioscience and BD Biosciences, respectively.
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2

Comprehensive Immune Phenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
TCR-β (H57–597), F4/80 (BM8), CD2 (RM2–5), CD3e (145–2C11), CD4 (GK1.5), CD8b (53–6.7), CD19 (eBio1D3), CD45R (B220, RA3–6B2), Gr-1 (Ly6G, RB6–8C5), Cd11b (Mac1, M1/70), Ter119 (Ly76) and NK1.1 (Ly53, PK136)-FITC were all from eBioscience and used for lineage determination. c-Kit (CD117, ACK2)-APCeFluor780 from eBioscience, Sca-1-Pacific blue from Biolegend, FcgRII/lll-PE (CD 16/32, 2.4G2), CD34 (RAM34)-AlexaFluor 647, CD135 (Flt3, A2F10)-PE, CD150 (Slamfl, TC15–12F12.2)-PECy7 were from Biolegend and used to quantify HSPCs and progenitor subsets. Peripheral leukocytes were stained with CD115 (AFS98)-APC, CD45 (30-F11)-APCCy7 and Ly6C/G or Gr-1 (RB6–8C5)-PercPCy5.5 from eBioscience and BD Biosciences, respectively.
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