Formic acid (>98% purity), acetonitrile (LC-MS grade), methanol, rutin, quercetin, apigenin and kaempferol were purchased from Merck (Darmstadt, Germany). apigenin 7-O-glucoside, quercetin 3-O-glucoside, kaempferol 3-O-rutinoside and luteolin were purchased from Extrasynthese (Genay Cedex, France). Cynarin, chlorogenic acid, 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, luteolin 7-O-glucuronide, apigenin, 7-O-glucoside, luteolin 7-O-glucoside and diosmetin were purchased from Phytolab (Vestenbergsgreuth, Germany). Bond Elut Jr 500 mg SPE-C18 cartridge was from Agilent (Santa Clara, California, USA). A Milli-Q purification system (Millipore, Bedford, MA, USA) was used to obtain the deionised water (18.2 MΩ cm). All chemicals used for biological assays were obtained as pure commercial products from Sigma Chemical Co (St. Louis, USA) and used without further purification. Spectrophotometric determinations were obtained with an Ultrospec 2100 spectrophotometer (Biochrom Ltd, Cambridge, England) using a 1 cm length path cell.
Bond elut jr 500 mg spe c18 cartridge
Manufactured by Agilent Technologies
Sourced in United States
The Bond Elut Jr 500 mg SPE-C18 cartridge is a solid-phase extraction (SPE) device designed for sample preparation. It contains 500 mg of C18 sorbent packed in a small cartridge format. The cartridge is intended for the extraction, purification, and concentration of analytes from liquid samples.
Automatically generated - may contain errors
Lab products found in correlation
Luteolin, by Extrasynthese (1 mentions)
Chlorogenic acid, by Phytolab (1 mentions)
Diosmetin, by Phytolab (1 mentions)
Apigenin, by Phytolab (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Apigenin, by Merck Group (1 mentions)
Quercetin 3 o glucoside, by Extrasynthese (1 mentions)
Ultrospec 2100 spectrophotometer, by Harvard Bioscience (1 mentions)
1 5 dicaffeoylquinic acid, by Phytolab (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
3 5 dicaffeoylquinic acid, by Phytolab (1 mentions)
4 5 dicaffeoylquinic acid, by Phytolab (1 mentions)
Cynarin, by Phytolab (1 mentions)
Luteolin 7 o glucuronide, by Phytolab (1 mentions)
Kaempferol 3 o rutinoside, by Extrasynthese (1 mentions)
Luteolin 7 o glucoside, by Phytolab (1 mentions)
Formic acid, by Merck Group (1 mentions)
Apigenin 7 o glucoside, by Extrasynthese (1 mentions)
Quercetin, by Merck Group (1 mentions)
2 protocols using bond elut jr 500 mg spe c18 cartridge
1
Phytochemical Analysis of Plant Extracts
2
Extraction and Purification of Phytochemicals
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Two extracts were prepared from each sample; 0.500 g of dried and ground powder was extracted using an ultrasonic bath (Soltec, Sonica S3 EP 2400) operating at 40 KHz with 10 mL of ethanol, 3 times for 10 min each. The supernatants were combined and centrifuged at 5000 rpm for 10 min, poured into a glass balloon, and evaporated in a rotary evaporator under vacuum at a temperature below 50 °C. In order to reduce chlorophyll interference, 30 mg of crude extract were resuspended in 1 mL of methanol/water (20 : 80, v/v), loaded onto a Bond Elut Jr 500 mg SPE-C18 cartridge (Agilent Technologies), eluted with 8 mL of methanol/water (95 : 5, v/v), and evaporated in a rotary evaporator. Pool samples, obtained by mixing the leaves that were harvested in summer (PGSum) and autumn (PGAut), were also created and extracted in the same way.
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