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2 protocols using l malic acid

1

Characterization of Red Raspberry Bioactives

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Red raspberries of the cultivar “Autumn Bless”, one abundant raspberry species in Northeast China, were obtained from a local farm (Harbin, China) in August 2020. The fruits with similar maturity were hand-picked from different trees and then snap-frozen. Frozen red raspberries (40 kg) were mixed, transported to the laboratory in ice box and stored at −80 °C until use. All the standards for high performance liquid chromatography (HPLC) analysis, including rutin, quercetin, chlorogenic acid, ellagic acid, raspberry ketone, epicatechin, catechinic acid, syringic acid, gallic acid, caffeic acid, luteolin, citric acid, L-malic acid, succinic acid, α-ketoglutarate, and so on (HPLC grade), were obtained from Shanghai YuanYe Biotechnology (Shanghai, China).
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2

Metabolic Profiling of TGF-β1-Induced EMT

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Tan IIA (purity > 98%), [U-13C]-glucose, l-serine, l-glycine, l-lactic acid, l-alanine, pyruvate, l-malic acid, succinate, fumarate, l-glutamic acid, citric acid, α-ketoglutaric acid, l-asparatic acid, and l-proline were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Recombinant human TGF-β1, Yefluor 488 EdU imaging kit, and cell cycle and apoptosis analysis kit were purchased from Yeasen (Shanghai, China). Sulforaphane (SFN), dimethyl sulfoxide (DMSO) and methoxyamine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). d-glucose was purchased from Aladdin (Shanghai, China). Dulbecco's modified Eagle medium (DMEM), minimum essential medium (MEM), and 0.25% trypsin were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) was purchased from Vazyme (Nanjing, China). SB 204990 was purchased from MedChemExpress (Princeton, NJ, USA). BTA was purchased from Macklin (Shanghai, China). Glucose assay kit was purchased from Elabscience (Wuhan, China). Lactate assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide was purchased from Acmec (Shanghai, China). Triton ×100, hoechst33342, antifade mounting medium and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime (Shanghai, China).
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