Nuclease p1 penicillium citrinum

MC was generously gifted by Professor Maria Tomasz. DMC was synthesized from MC by a published procedure.^{[47 (link)]} Phosphodiesterase I (snake venom diesterase (SVD), Crotalus adamanteus venom, E.C. 3.1.4.1.) and alkaline phosphatase (Escherichia coli, EC 3.1.3.1) were obtained from Worthington Biochemical Corp (Freehold, NJ). Nuclease P₁ (penicillium citrinum, EC 3.1.30.1) was from Sigma Life Sciences (St. Louis, MO). Sep-Pak C-18 cartridges were purchased from Waters Corp (Milford, MA). Oligonucleotides were purchased from Midland Certified Reagent (Midland, TX).

Reduced glutathione (GSH), oxidized glutathione GSSG, O-(pyridin-3-ylmethyl)hydroxylamine (PMOA), butyraldehyde, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), calf thymus (CT) DNA, p-Benzoquinone (p-BQ), HQ, 2-methylhydroquinone (2-MeHQ), Cat, p-cresol (p-Cre), m-cresol (m-Cre), and o-cresol (o-Cre), Acr, methylacrolein (MeAcr), crotonaldehyde (Crot), Methyl vinyl ketone (MVK), RNase A (bovine pancreas), RNase T1 (Aspergillus oryzae), proteinase K (Tritirachium album), sodium dodecyl sulfate (SDS), DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), and nuclease P1 (Penicillium citrinum) were purchased from Sigma Aldrich (St. Louis, MO, USA). Phosphodiesterase I (Crotalus adamanteus venom) and adenosine deaminase were purchased from Worthington Biochemical Corp. (Newark, NJ). LC/MS grade solvents were purchased from Fisher Chemical Co. (Pittsburgh, PA). Puregene protein precipitation solution was purchased from Qiagen (Germantown, MD). Strata-X 33 μm Polymeric Reversed Phase 30 mg solid-phase extraction (SPE) cartridge was obtained from Phenomenex (Torrance, CA, USA). If not stated otherwise, all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).