Diethylnitrosamine (DEN, 73861), CCl4 (1601168), Oligomycin (O4876), Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920), antimycin A (A8674), rotenone (R8875), 2-deoxy-d -glucose (2-DG, D8375), PNGase F (G5166) and Swainsonine (S9263) and D-(+)-Glucose (G7021) were obtained from Sigma (St. Louis, MO, USA). SYBR green was purchased from Bio-Rad (Hercules, CA, USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse immunoglobulin G were obtained from Beyotime (Haimen, Jiangsu, China). Glucose deficient DMEM medium and Trizol Reagent were obtained from Life Technologies (Carlsbad, CA, USA). Tunicamycin (B7417) was obtained from ApexBio Technology (Houston, TX, USA). UNC-2250 (S7342) was purchased from Selleck Chemicals (Houston, TX, USA). The antibodies used in this study are: MerTK (abcam; ab52968), Phospho-MerTK (abcam; ab14921), Akt (Cell Signaling Technology; 4691), Phospho-Akt (Ser473) (Cell Signaling Technology; 4060), Phospho-GSK3β (Cell Signaling Technology; 5558S), PGK1 (ZN; 501965), LDHA (ZN; 501146), PFKM (ZN; 505477), PKM2 (ZN; 505477), PDHK1 (Cell Signaling Technology; 3820S), Lamin B (Proteintech; 12987-1-AP), GAPDH (Abmart; M20028) and β-Actin (Proteintech; 60008-1-1 g).
Swainsonine s9263
Manufactured by Merck Group
Sourced in United States
Swainsonine (S9263) is a chemical compound that serves as a laboratory reagent. It is a glycosidase inhibitor and can be used for various research applications. The core function of Swainsonine is to inhibit specific glycosidase enzymes, which are involved in the processing and modification of carbohydrates. This inhibition can be useful in the study of cellular processes, glycoprotein synthesis, and other areas of biochemical research.
Automatically generated - may contain errors
Lab products found in correlation
Oligomycin, by Merck Group (1 mentions)
Lamin b, by Proteintech (1 mentions)
Phospho gsk3β, by Cell Signaling Technology (1 mentions)
Unc 2250, by Selleck Chemicals (1 mentions)
Phospho mertk, by Abcam (1 mentions)
Pdhk1, by Cell Signaling Technology (1 mentions)
Mertk, by Abcam (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
D glucose, by Merck Group (1 mentions)
Diethylnitrosamine, by Merck Group (1 mentions)
β actin, by Proteintech (1 mentions)
Sybr green, by Bio-Rad (1 mentions)
Gapdh, by Abmart (1 mentions)
Tunicamycin, by Apexbio (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
2 protocols using swainsonine s9263
1
Evaluating Metabolic Reprogramming Mechanisms
2
Immunohistochemical Analysis of CD147 and N-Glycans in HCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibody details are listed in the supplementary material, Table S1 . Rabbit anti‐CD147 and α‐tubulin were developed in our laboratory 26 . Formalin‐fixed, paraffin‐embedded tissues from HCC patients were stained with HAb18G antibody against CD147 (30 μg/ml) and PHA‐L (1:200) for β1,6‐branched N‐glycans as previously described 15 . The PI3K inhibitor LY294002 was purchased from Cell Signaling Technology (Danvers, MA, USA). TGF‐β1 (100‐210) was purchased from PeproTech (Rocky Hill, NJ, USA) and swainsonine (s9263) from Sigma‐Aldrich (St Louis, MO, USA).
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