Hif1α sirna sc 35561

miR-338-3p mimic, miR-338-3p inhibitor, miR-338-3p mutant and negative control (NC) were purchased from Shanghai Gene-Pharma Co. (Shanghai, China). HIF1α-siRNA (sc-35561) and control siRNA-A (sc-37007) were purchased from Santa Cruz Biotechnology, Inc. HIF1α plasmid (18949) [21] (link) and hypoxia response element (HRE)-luc pGL vector (26731) [22] (link) were purchased from Addgene (Cambridge, MA). Control pGL vector were obtain from Promega (Madison, WI). The 3′UTR of HIF1A was PCR-amplified from HepG2 cDNA and cloned downstream of the luciferase gene in the pGL vector (Promega). Plasmid, siRNA, and miRNA transfection was performed using Lipofectamine 2000 (Invitrogen). Cells were subjected to functional or mechanistic analyses two days post-transfection. Luciferase activity was measured using the dual-luciferase reporter system (Promega). Renilla activity was used to normalize the relative firefly luciferase values.