All chemicals were from Sigma-Aldrich (Buchs, Switzerland), Sigma Chemical Co. (St. Louis, MI, USA) and Merck KGaA (Darmstadt, Germany). Sodium phosphate monobasic was from Fluka Analytical (Darmstadt, Germany). The cell reagents were provided by Life Technologies, S.A. (Madrid, Spain) and Biowest (Nuaillé, France). Human foreskin fibroblasts (HFF-1) were obtained from ATCC (Manassas, VI, USA). Human immortalized nontumorigenic keratinocytes cell line (HaCaT) was acquired from CLS Cell Lines Service (Eppelheim, Germany). Elastatinal inhibitor and human neutrophil elastase were from Enzo Life Sciences (Farmingdale, NY, USA).
Elastatinal inhibitor
Manufactured by Enzo Life Sciences
Sourced in United States
Elastatinal is a specific inhibitor of the serine protease enzyme elastase. It functions by binding to and blocking the active site of elastase, preventing it from carrying out its catalytic activity.
Automatically generated - may contain errors
Lab products found in correlation
Human foreskin fibroblasts hff 1, by American Type Culture Collection (1 mentions)
Kojic acid, by Merck Group (1 mentions)
Butylhydroxytoluene bht, by Merck Group (1 mentions)
Fecl3, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Ethyl acetate, by Avantor (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
Ethanol, by Avantor (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
L dopa, by Merck Group (1 mentions)
2 protocols using elastatinal inhibitor
1
Human Cell Lines Culture Protocol
2
Evaluation of Grapefruit and Strawberry Bioactivities
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The sample of grapefruit (C. Maxima L.) was obtained from Bantul Residence, Yogyakarta, Indonesia. The ripped strawberry sample was obtained from strawberry farm in Bandung, West Java, Indonesia. Chemicals used in this research include: Ethanol (JT-Baker, USA), ethyl acetate (JT-Baker, USA), dimethyl sulfoxide (DMSO), 10% SDS, HCl, 28 N-succinyl-(ala) 3-p-nitroanilide (Sigma, USA) and buffers Tris-Cl (Sigma, USA), tyrosinase (mushroom) enzyme (sigma, USA), enzyme Neutrophil Human Elastase (Enzo Life Science, USA), elastatinal inhibitor (Enzo Life Science, USA), MeOSuc-AAPV-pNa substrate, enzyme Matrix Metalloproteinase-1 (MMP-1, Enzo Life Science, USA)), MMP-substrate (BML-P125-9090) (Enzo Life Science, USA), NNGH inhibitors (Enzo Life Science, USA), kojic acid, ascorbic acid (Merck, Germany), L-Dopa, iron (II) sulphate (FeSO4. 7H2O) (sigma, USA), 2,4,6-tripiridyl-s-triazine (TPTZ) (sigma, USA), FeCl3 (sigma, USA), linoleic acid, Tween 20 (sigma, USA), β-carotene (sigma, USA), butyl hydroxy toluene (BHT) (sigma, USA), sodium acetate.
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