We first examined FGF8 at different doses (2, 10, 20, and 100 ng/mL) and time periods, and FGF21 (10 ng/mL, AF10042; PeproTech). Next, the additive effect of various small molecules including kisspeptin (0.1 µM; Phoenix Pharm., Burlingame, CA, USA), Sonic Hedgehog (SHH, 20 ng/mL; Fisher), and retinoic acid (RA; 3 ng/mL; Fisher), with or without FGF8, was examined. Additionally, the effects of 2 notch inhibitors were examined: compound E (0.5 ng/mL; Fisher), DAPT (20µM; Sigma), and the antimitotic drug fluorodeoxyuridine (FdUR; 30 µM; Sigma). The doses of DAPT and FdUR were the same or 50% lower as those described for stem cell–derived GnRH cultures (27 (link),28 (link)) and for primary cultures of GnRH neurons (8 (link),17 (link)), respectively.
Compound e
Manufactured by Thermo Fisher Scientific
Compound E is a laboratory reagent provided by Thermo Fisher Scientific. It is a chemical compound that can be used in various research and analytical applications. The core function of Compound E is to serve as a tool for scientific investigation and experimentation, without further interpretation of its intended use.
Automatically generated - may contain errors
Lab products found in correlation
4 aminophenylmercuric acetate apma, by Merck Group (1 mentions)
U2os cell, by American Type Culture Collection (1 mentions)
Batimastat bb94, by Merck Group (1 mentions)
Decma 1 antibody, by Merck Group (1 mentions)
Mmp 2, by R&D Systems (1 mentions)
Recombinant dll4, by R&D Systems (1 mentions)
Mmp 9, by R&D Systems (1 mentions)
2 protocols using compound e
1
Modulation of GnRH Neuron Development
2
DLL4, MMP-2, and MMP-9 Regulation in U2OS Cells
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Recombinant DLL4, MMP-2, and MMP-9 were purchased from R and D Systems. Batimastat (BB-94) was purchased from Sigma Aldrich. Compound E (GSI) was purchased from Fisher Scientific (Catalog # AAJ65131EXD). DECMA-1 antibody was purchased from Sigma-Aldrich (Cat# U3254, RRID:AB_477600 ). Z-DEV-FMK was purchased from R and D Systems (FMK004). E-cadherin primary antibody was purchased from Thermo Fisher Scientific (Cat# 33–4000, RRID:AB_2533118 ). β-tubulin primary antibody was purchased from Sigma-Aldrich (Cat#T8328, RRID:AB_1844090 ). U2OS cells were purchased from ATCC (Cat# HTB-96, RRID:CVCL_0042 ). MS5 and MS5-DLL4 cells were a kind gift from Dr. Stephen Blacklow. 4-aminophenylmercuric acetate (APMA) was purchased from Sigma-Aldrich. Herceptin was purchased from MedChemExpress (HY-P9907).
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