NF-κB oligonucleotide: Promega, Mannheim, Germany; [γ33P]ATP: Hartmann Analytic, Braunschweig, Germany; T4 polynucleotide kinase: New England Biolabs, Frankfurt, Germany; William's medium E, L-glutamine and DMEM: Biochrome, Berlin, Germany; LightCycler 480 Probes Master, penicillin-streptomycin, poly(dI-dC), cOmplete EDTA free protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, 0.45 µm PVDF membrane and Cell Death Detection ELISAPLUS Kit: Roche, Mannheim, Germany; Quantikine Cytochrome c ELISA, recombinant IL-1β and recombinant TNFα: R&D Systems, Minneapolis, USA; RNeasy Plus Mini Kit and QuantiTect Reverse Transcription Kit: Qiagen, Hilden, Germany; primer and probes for quantitative RT-PCR 2: Sigma, Steinheim, Germany; actinomycin D (ActD): Enzo Life Sciences, Lörrach, Germany; p38 inhibitor RN3503 and JNK inhibitor SP600125: Prof. S. Laufer, University of Tübingen, Germany; Bradford Quick Start Dye: Bio-Rad Laboratories, Munich, Germany; ECL western blotting detection reagents, GE Healthcare, Little Chalfont, UK; pJNK, JNK, PARP, XIAP, A20/TNFAIP3, α-tubulin, pro-caspase-3 and cleaved caspase-3 antibodies: Cell Signaling, Danvers, USA; horseradish peroxidase conjugated anti-rabbit and anti-mouse secondary antibodies: Jackson Immuno Research, Newmarket, UK.
Nf κb oligonucleotide
Manufactured by Promega
Sourced in Germany
The NF-κB oligonucleotide is a synthetic DNA sequence that corresponds to the binding site for the transcription factor NF-κB. It can be used in various laboratory techniques, such as electrophoretic mobility shift assays (EMSAs) and ChIP-seq, to study the DNA-binding activity and regulation of NF-κB.
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Lab products found in correlation
Ecl western blotting detection reagent, by GE Healthcare (1 mentions)
Procaspase 3, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
A20 tnfaip3, by Cell Signaling Technology (1 mentions)
γ 33p atp, by Hartmann Analytic (1 mentions)
Horseradish peroxidase conjugated anti rabbit and anti mouse secondary antibodies, by Jackson ImmunoResearch (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
P jnk jnk, by Cell Signaling Technology (1 mentions)
T4 polynucleotide kinase, by New England Biolabs (1 mentions)
Quantitect reverse transcription kit, by Qiagen (1 mentions)
Rneasy plus mini kit, by Qiagen (1 mentions)
Cell death detection elisaplus kit, by Roche (1 mentions)
Lightcycler 480 probes master, by Roche (1 mentions)
2 protocols using nf κb oligonucleotide
1
NF-κB Activation and Apoptosis Assay
2
NF-κB Activation Assay in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RINm5F cells were pretreated with varying doses of celastrol for 1 h, and then stimulated with cytokines (5 ng/ml IL-1β, 10 ng/ml TNF-α, and 10 ng/ml IFN-γ) for 30 min. Nuclear extracts were prepared, and the EMSA was performed as described previously (24) (link). Nuclear extracts (10 μg) were incubated with 32P-end labeled double-stranded NF-κB oligonucleotide (5'-AGT TGA GGG GAC TTT CCC AGG C-3'; Promega) in binding buffer (10 mM Tris-HCl, pH 8.0, 75 mM KCl, 2.5 mM MgCl2, 0.1 mM EDTA, 10% glycerol, 0.25 mM DTT, and 1 μg of poly dI/dC) for 30 min. The DNA-protein complexes were analyzed by electrophoresis on a 6% native polyacrylamide gel in TBE buffer (89 mM Tris-HCl, 89 mM boric acid, and 2 mM EDTA). Then, the gels were dried and examined by autoradiography.
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