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2 protocols using anti lmnb1 101237 t32

1

Protein Expression and Antibody Validation

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Anti-β-actin (A1978), horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-mouse-IgG-HRP (AP308P), and anti-rabbit-IgG-HRP (AP132P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). Anti-c-Myc (HT101) was purchased from TransGen Biotech (Beijing, China). Anti-TAK1 (5206) and p65 (8242) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). Anti-CHIP (sc-133066) was purchased from Santa Cruz Biotechnology (San Diego, CA, USA). TNFα Recombinant Human Protein (10602HNAE25) was purchased from Thermo Fisher (Waltham, MA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from SHSY5Y cDNA and then subcloned into the pcDNA3.1 vector. The recombinant plasmids were confirmed by DNA sequencing in Sangon Biotech (Shanghai, China).
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2

Wnt-3a Signaling Pathway Regulation

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Recombinant Human Wnt-3a (NP-149122) was purchased from an R&D system (Minneapolis, MN, USA).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).
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