Anti hmgcr sc 271595

The statins (mevalonate pathway inhibitors) used were simvastatin (SIM, Sigma-Aldrich, Hamburg, Germany), rosuvastatin calcium (ROSU, SelleckChem, Munich, Germany), and atorvastatin calcium salt trihydrate (ATO, Sigma-Aldrich, Hamburg, Germany). Stocks were prepared in DMSO. Primary antibodies for Western Blot analyses were: anti-Rap1A (sc-1482; recognizes the ungeranylated Rap1A^{47 (link)}), anti-HMGCR (sc-271595) from Santa Cruz (Heidelberg, Germany), anti-HMGCR (ab214018) from Abcam (Cambridge, UK), anti-cleaved PARP (#9541) from Cell Signaling Technology, Inc. (Beverly, MA, USA), and anti-SREBP2 (#557037) from BD Biosciences (Heidelberg, Germany). For GAPDH, we used the anti-GAPDH (sc-25778) from Santa Cruz (Heidelberg, Germany) and anti-GAPDH (#5G4) from HyTest Ltd. (Turku, Finland). Secondary horseradish peroxidase-conjugated antibodies were anti-mouse IgG (HAF007), anti-rabbit IgG (HAF008), and anti-goat IgG (HAF109) from R&D Systems, Inc. (Minneapolis, MN, USA).

The applied antibodies were as follows: anti‐BRCC36 (15391‐1‐AP), anti‐Ubiquitin (10201‐2‐AP), anti‐NLRP3 (19771‐1‐AP), and anti‐GSDMD (20770‐1‐AP) were purchased from Proteintech (Rosemont, Illinois, USA); anti‐HMGCR (sc‐271595) was purchased from Santa Cruz (Dallas, Texas, USA); anti‐cleaved‐GSDMD (#36 425), anti‐BRCC36 (#18215S), and Normal Rabbit IgG (#2729) were purchased from Cell Signaling Technology; anti‐HMGCR (ab174830), anti‐cleaved caspase‐1 (ab207802), and anti‐4‐Hydroxynonenal (ab46545) were purchased from Abcam. Anti‐β‐actin (A5441) and anti‐Flag (F1804) were purchased from Sigma‐Aldrich. Anti‐HMGCR (A19063) was purchased from ABclonal. The reagents used in the experiment were as follows: RSL3 (S8155) was obtained from Selleck; Ferrostatin‐1 (SML0583) and Lithium phosphorus sulfide (LPS) (L2630) were purchased from Sigma‐Aldrich; Thiolutin (THL) and 25‐hydroxycholesterol were obtained from APExBIO.