A standardized questionnaire adapted from a Family Health International (FHI) 360 validated guide for bio-behavioral surveys was administered during a face-to face interview to collect information regarding socio-demographic characteristics, risky sexual behaviors.
Cervical and anal swabs were collected respectively from FSW and MSM with Cervex-Brush® Combi and Anex-Brush®, respectively (Rovers Medical Devices, Oss, the Netherlands) and conserved in preservative solution (BDSurePath™ liquid-based Papanicolaou tests, Becton-Dickinson, Franklin Lakes, NJ, USA). These samples were stored at 4 °C and sent at room temperature to France for analyses. Nucleic acids were extracted using a STARMag 96 × 4 Universal Cartridge kit (Seegene, Seoul, Korea) on the MICROLAB NIMBUS automat (Seegene). T. vaginalis and six other STI (N. gonorrhoeae, C. trachomatis, M. genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum) were identi ed using the Allplex STI Essential Assay (Seegene).
Venous blood samples were collected to test for HIV and Syphilis using SD Biolane Duo (Abbott). Each HIV positive case was con rmed with a second rapid diagnosis test, First Response HIV 1-2-O Card Test (Premier Medical Corporation Pvt. Ltd). In case of discordant results, samples were performed with the INNO-LIA HIV I/II Score (20T) (Fujirebio) line immunoassay (Fujirebio, Göteborg, Sweden).
Cervical and anal swabs were collected respectively from FSW and MSM with Cervex-Brush® Combi and Anex-Brush®, respectively (Rovers Medical Devices, Oss, the Netherlands) and conserved in preservative solution (BDSurePath™ liquid-based Papanicolaou tests, Becton-Dickinson, Franklin Lakes, NJ, USA). These samples were stored at 4 °C and sent at room temperature to France for analyses. Nucleic acids were extracted using a STARMag 96 × 4 Universal Cartridge kit (Seegene, Seoul, Korea) on the MICROLAB NIMBUS automat (Seegene). T. vaginalis and six other STI (N. gonorrhoeae, C. trachomatis, M. genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum) were identi ed using the Allplex STI Essential Assay (Seegene).
Venous blood samples were collected to test for HIV and Syphilis using SD Biolane Duo (Abbott). Each HIV positive case was con rmed with a second rapid diagnosis test, First Response HIV 1-2-O Card Test (Premier Medical Corporation Pvt. Ltd). In case of discordant results, samples were performed with the INNO-LIA HIV I/II Score (20T) (Fujirebio) line immunoassay (Fujirebio, Göteborg, Sweden).