Rpmi 1640 culture medium

Ginsenoside CK was obtained from Xi’an XiaoCao Botanical Development Co., Ltd. Both PC and DP were purchased from Shanghai Advanced Vehicle Technology Pharmaceutical Co., Ltd., Shanghai, China. Coumarin-6, 1,1′-dioctadecyl- 3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Shanghai Aladdin Bio-Chem Technology Co. Ltd, Shanghai, China. A549 cells, RPMI 1640 culture medium, fetal bovine serum, as well as anti-caspase 3, anti-caspase 8, anti-caspase 9, anti-poly ADP-ribose polymerase (anti-PARP), anti-Bax, and anti-Bcl-2 antibodies were obtained from Jiangsu Keygen Biotech Corp., Ltd., Nanjing, China. Nude mice were purchased from Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China. The other solvents used were of chromatographic grade.

All initial reagents were obtained commercially and used as received. 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide (EDC), dimethyl sulfoxide (DMSO) and sulfo-NHS (N-hydroxysulfosuccinimide), and Ce6 were obtained from Adamas-beta (Shanghai, China). NH₂-mPEG-NH₂(2k) was obtained from ToYong Bio Co., Ltd. (Shanghai, China). Singlet Oxygen Sensor Green (SOSG) was purchased from Molecular Probes (Eugene, USA). Trypsin-EDTA (0.25%) was from New Cell & Molecular Biotech Co., Ltd. (Suzhou, China). PI, DAPI, CCK-8, RPMI-1640 culture medium, DMEM/F12 culture medium, and DMEM were from KeyGEN BioTECH (Jiangsu, China). The DCFH-DA Kit was from Beyotime (Shanghai, China). Human head and neck squamous cells, HSC3, CAL27, SCC4, and FaDu, were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, P. R. China).