Xn 1000 automated hematology analyzer

After the first admission and following a 12-hour fast, blood samples were obtained from the antecubital vein. Full blood counts were analyzed within 45 minutes of obtaining blood sample. The venous blood samples obtained for biochemical analysis were centrifuged at 3000 rpm. They were then stored at −80°C until analysis. Biochemical analyses were performed with an Architect Ci8200 (Abbott Laboratories, Lake Bluff, IL, USA), and full blood counts were performed with an XN-1000 Automated Hematology Analyzer (Sysmex, Tokyo, Japan). All analyses were performed by a laboratory technician who was blinded to the patient data. The following parameters were measured during the biochemical analysis: fasting blood glucose, creatinine, albumin, total protein, sodium, potassium, blood urea nitrogen, aspartate transaminase, alanine transaminase, uric acid, calcium, C-reactive protein (CRP), thyroid-stimulating hormone, total cholesterol, low-density lipoprotein, very-low-density lipoprotein, high-density lipoprotein, and triglycerides. The full blood count included measurement of the red blood cell count, hemoglobin, hematocrit, red cell distribution width (RDW), platelet count, platelet distribution width, WBC count, neutrophil count, and lymphocyte count. The ratio of the neutrophil count over the lymphocyte count was recorded as the NLR.

Red blood cell, white blood cell, and platelet counts were measured by flow
cytometry using Sysmex XN-1000 Automated Hematology Analyzer (Sysmex Corp,
Kobe, Japan). C-reactive protein (CRP), fibrinogen, activated partial
thromboplastin time (APTT), PT, and d-dimer were measured using the
following commercial kits: CRP-Latex X2 (Denka Seiken Co, Ltd, Niigata,
Japan), Thrombocheck Fib(L) (MBL Co, Ltd, Ina, Japan), Thrombocheck APTT-SLA
(Sysmex Corp), Thromborel S (Sysmex Corp), and Hexamate D-dimer (MBL Co,
Ltd), respectively.