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Ssea 3

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SSEA-3 is a laboratory equipment used to detect and measure the expression of the SSEA-3 antigen, a cell surface marker commonly associated with embryonic stem cells and other undifferentiated cell types. The core function of SSEA-3 is to provide researchers with a tool to analyze and characterize the phenotypic properties of cell populations in various research applications.

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Lab products found in correlation

Nanog, by Abcam (2 mentions) Alexa fluor 488 or 594, by Thermo Fisher Scientific (2 mentions) Smi 32, by Fortrea (2 mentions) Neurofilament 200, by Merck Group (1 mentions) Hnrnp q, by Merck Group (1 mentions) Nrxn 2α, by Abcam (1 mentions) Lcs2 confocal microscope, by Leica camera (1 mentions)

2 protocols using ssea 3

1

Immunocytochemical Characterization of Stem Cells

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Cells were fixed in 4% paraformaldehyde for 10 min, permeabilized with Triton 0.25%, and then blocked with 10% bovine serum albumin in 1× phosphate-buffered saline and 0.3% Triton X-100 for 1 h at room temperature. We incubated the cells with primary antibodies to NANOG (1:100, Abcam), SSEA-3 (1.100, Covance), ChAT (1:200, Chemicon & Millipore), SMI32 (Covance, 1:500), and Neurofilament-200 (1:1000 Sigma) overnight, and then with anti-rabbit or anti-mouse or -goat Alexa Fluor 488 or 594 (1:400; Life Technologies) secondary antibody for 1.5 h at room temperature.
Rizzo F., Ramirez A., Compagnucci C., Salani S., Melzi V., Bordoni A., Fortunato F., Niceforo A., Bresolin N., Comi G.P., Bertini E., Nizzardo M, & Corti S. (2017). Genome-wide RNA-seq of iPSC-derived motor neurons indicates selective cytoskeletal perturbation in Brown–Vialetto disease that is partially rescued by riboflavin. Scientific Reports, 7, 46271.
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2

Immunocytochemical Characterization of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde for 10 min, permeabilized with Triton™ 0.25%, and then blocked with 10% bovine serum albumin in phosphate-buffered saline and 0.3% Triton™ X-100 for 1 h at room temperature. We incubated the cells with primary antibodies to NANOG (1:100, Abcam), SSEA-3 (1.100, Covance), ChAT (1:200, Chemicon and Millipore), SMI32 (1:500, Covance), hnRNP-Q (1:100, Sigma), and NRXN 2α (1:100, Abcam) overnight, and then with anti-rabbit, anti-mouse, or anti-goat Alexa Fluor® 488 or 594 (1:400; Life Technologies) secondary antibody for 1.5 h at room temperature. Negative controls were performed for all stains. Microphotographs were taken with a LEICA LCS2 confocal microscope.
Rizzo F., Nizzardo M., Vashisht S., Molteni E., Melzi V., Taiana M., Salani S., Santonicola P., Di Schiavi E., Bucchia M., Bordoni A., Faravelli I., Bresolin N., Comi G.P., Pozzoli U, & Corti S. (2019). Key role of SMN/SYNCRIP and RNA-Motif 7 in spinal muscular atrophy: RNA-Seq and motif analysis of human motor neurons. Brain, 142(2), 276-294.
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