Dioleoylphosphatidylserine dops

Manufactured by Avanti Polar Lipids

Sourced in United States

Dioleoylphosphatidylserine (DOPS) is a synthetic phospholipid compound. It is a key component of cell membranes and is involved in various cellular processes. DOPS serves as a versatile tool for research applications in biochemistry and cell biology.

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Lab products found in correlation

Dioleoylphosphatidylcholine dopc, by Avanti Polar Lipids (1 mentions) 1 1 2 2 tetraoleoyl cardiolipin tocl, by Avanti Polar Lipids (1 mentions) Dioleoylphosphatidylethanolamine dope, by Avanti Polar Lipids (1 mentions) Sephadex g25 column pd 10, by Cytiva (1 mentions)

3 protocols using dioleoylphosphatidylserine dops

Saposin C Liposome Preparation

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Example 2

Dioleoylphosphatidylserine (DOPS) was obtained from Avanti Polar Lipids (Alabaster Ala.). Twenty to thirty μmoles of DOPS in chloroform were dried under N₂and vacuum to lipid films. Five to ten μmoles saposin C polypeptide was added to the dried films and suspended in 50 μl McIlvanine buffer (pH 4.7). The suspension was then brought to a 1 ml volume with either cell culture medium or phosphate buffered saline (PBS) (Ausubel et al. (2002) Current Protocols in Molecular Biology. John Wiley & Sons, New York, N.Y., herein incorporated by reference). The mixture was sonicated in a bath sonicator for approximately 20 minutes. Ice was added as needed to prevent overheating the samples.

US10188698B2. Saposin C-DOPS: a novel anti-tumor agent (2019-01-29). Children's Hospital Medical Center [US]. Inventors: Xiaoyang Qi [US].

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Lipid Standards Procurement and Characterization

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All reagents used were purchased from Sigma Chemical Co. (St. Louis, MO). Phospholipid standards including dioleoylphosphatidylserine (DOPS), dioleoylphosphatidyl-ethanolamine (DOPE), dioleoylphosphatidylcholine (DOPC) and 1,1’,2,2’-tetraoleoylcardiolipin (TOCL) were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Stoyanovsky D.A., Sparvero L.J., Amoscato A.A., He R.R., Watkins S., Pitt B.R., Bayir H, & Kagan V.E. (2014). Improved Spatial Resolution of MALDI Imaging of Lipids in the Brain by Alkylated Derivatives of 2,5-Dihydroxybenzoic Acid. Rapid communications in mass spectrometry : RCM, 28(5), 403-412.

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Preparation of Fluorescent SapC-DOPS Nanovesicles

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Dioleoylphosphatidylserine (DOPS; Avanti Polar Lipids Inc., Alabaster, AL) was dried under a stream of N₂ gas, combined with recombinant SapC protein (27 (link)) in citrate/phosphate buffer (pH 5.0) and bath sonicated in PBS as described in detail previously (21 (link)). To fluorescently label SapC-DOPS nanovesicles, an aliquot of CellVue® Maroon (CVM, Molecular Targeting Technology Inc., Exton, PA) in ethanol was dried together with DOPS before SapC addition and bath sonication. Free CVM was eliminated from this preparation by filtration through a Sephadex^™ G25 column (PD-10, Amersham Pharmacia Biotech, Piscataway, NJ). For in vivo treatment, a lyophilized formulation was used: dry DOPS was suspended in 80% tert-butanol. SapC and sucrose (10 mg/ml) were dissolved in water. DOPS suspension and SapC plus sucrose (1:0.6, vol:vol) was lyophilized in a freeze dryer (VirTis Unitop 1000L linked to a Freezemobile 25XL; The VIRTIS, Gardiner, NY). The stable powder cake was resuspended in saline solution to form SapC-DOPS nanovesicles. Vesicles were monitored with a submicron particle size analyzer (Coulter Model N4 Plus; Fullerton, CA). The molar ratio of SapC:DOPS was 1:7.

Zhao S., Chu Z., Blanco V.M., Nie Y., Hou Y, & Qi X. (2015). SapC-DOPS Nanovesicles as Targeted Therapy for Lung Cancer. Molecular cancer therapeutics, 14(2), 491-498.

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