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β glccer

Manufactured by Avanti Polar Lipids
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Sourced in United States

β-GlcCer is a laboratory product offered by Avanti Polar Lipids. It is a glycosphingolipid that functions as a key structural component in cell membranes.

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Lab products found in correlation

Tyloxapol, by Merck Group (1 mentions) α galcer, by Alexis Biochemicals (1 mentions) Sulfatide, by Avanti Polar Lipids (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) α galcer krn7000, by Avanti Polar Lipids (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions)

4 protocols using β glccer

1

Lipid Characterization and CD1d Loading

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C24:1 (PBS44) was kindly provided by P. Savage (Brigham Young University). α-GalCer C26:0 was supplied by Alexis Biochemicals, and sulfatide (C24:1), β-GalCer (C12) and β-GlcCer (C24:1) were purchased from Avanti Polar Lipids. Disialo-ganglioside GD3 was purchased from Matreya. α-GlcCer (C20:2), α-GalCer (C20:2 analogue), and OCH were produced in house (at the University of Birmingham, UK). α-GalCer (C26:0 3′,4″-dideoxy- ‘3′-deoxy-α-GalCer' and C26:0 4′,4″-dideoxy ‘4′-deoxy-α-GalCer' analogues) were produced in house (at the University of Connecticut)47 (link). Lipids were dissolved in 0.5% v/v Tyloxapol (Sigma), or buffer containing 0.5% v/v tween-20, 57 mg ml−1 sucrose and 7.5 mg ml−1 histidine, and loaded into CD1d at a three to sixfold molar excess overnight.
Le Nours J., Praveena T., Pellicci D.G., Gherardin N.A., Ross F.J., Lim R.T., Besra G.S., Keshipeddy S., Richardson S.K., Howell A.R., Gras S., Godfrey D.I., Rossjohn J, & Uldrich A.P. (2016). Atypical natural killer T-cell receptor recognition of CD1d–lipid antigens. Nature Communications, 7, 10570.
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2

Activating iNKT Cells in Mice

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Bone marrow cells from femurs of mice were cultured for 7 days (5×106 cells/well) at 37°C in 6-well cell culture dishes with complete RPMI medium in the presence of recombinant murine GM-CSF (50 ng/ml, PeproTech) and IL-4 (10 ng/ml, PeproTech). On day six, cells were pulsed with either 100 ng – 1 ug/ml of αGalCer (KRN7000, Avanti Polar Lipids), 1 ug/ml OCH (Alexis Biochemicals), 1 ug/ml of βGlcCer (C24:1 Glucosyl(β) Ceramide (d18:1/24:1(15Z)), Avanti Polar Lipids), 1ug/ml of iGb3 provided by D. Zhou (MD Anderson) or 1 ul/ml solvent (2:1 methanol:chloroform) for 12-15 hours at 37 C. BMDCs were intravenously injected into Nur77gfp mice (0.5–1.0×106cells/mouse) and endogenous splenic iNKT cells were analyzed 16 h later.
Holzapfel K.L., Tyznik A.J., Kronenberg M, & Hogquist K.A. (2014). Antigen-dependent versus -independent activation of iNKT cells during infection. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5490-5498.
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3

Glycolipid Stimulation of iNKT Cells

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Total splenocytes or sorted iNKT cells (CD1d tetramer+ TCRβ+) were cultured in RPMI-1640 media containing 10% fetal calf serum, 25 mM HEPES, pH 7.2, 1% penicillin–streptomycin–glutamine and 55 μM β-mercaptoethanol. Total splenocytes were treated with αGalCer (100 ng ml−1), LPS (10 μg ml−1), GSL1 (10 μg ml−1; NIH Tetramer Core Facility, Atlanta, GA, USA), OCH (10 μg ml−1; NIH Tetramer Core Facility) and βGlcCer (10 μg ml−1; Avanti Polar Lipids). For the analysis of proliferation, cells were stained with CellTrace violet (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol.
Stradner M.H., Cheung K.P., Lasorella A., Goldrath A.W, & D’Cruz L.M. (2016). Id2 regulates hyporesponsive invariant natural killer T cells. Immunology and cell biology, 94(7), 640-645.
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4

Synthesis and Characterization of Glycosyl Sphingosines

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β-GlcCer, β-Gb3 sphingosine, and β-iGb3 sphingosine were purchased from Avanti Polar Lipids (Alabaster, USA). Synthesis routes of the investigated glycosyl (phyto-)sphingosines5 (link) and α-Gb3Cer35 (link) were described previously. α-GalCer and β-GalCer36 (link), α-GlcCer36 (link), α-Gal diacylglycerol37 and β-Gal diacylglycerol38 (link) were synthesized by following published procedures and adapting the lipid residues. 100 µM and 10 µM solutions of each glycolipid were prepared for obtaining IR spectra and ion mobility data, respectively. β-Gb3- and β-iGb3 sphingosine were dissolved in pure methanol. α-Gb3Cer was dissolved in dimethyl sulfoxide and diluted with methanol. The other glycolipids were dissolved in dimethyl sulfoxide (1–15 mM) and diluted in a 1:1 (v:v) mixture of acetonitrile and chloroform to obtain 1 mm stock solutions. Prior to measurements, the stock solutions were diluted in a 2:2:1 (v:v:v) mixture of acetonitrile, methanol and water. All solvents were purchased from Sigma-Aldrich. The solutions were stored at −32 °C.
Kirschbaum C., Greis K., Mucha E., Kain L., Deng S., Zappe A., Gewinner S., Schöllkopf W., von Helden G., Meijer G., Savage P.B., Marianski M., Teyton L, & Pagel K. (2021). Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy. Nature Communications, 12, 1201.
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