Log In Sign up
The largest database of trusted experimental protocols

Tskgel alpha columns

Manufactured by Tosoh
Visit Supplier
Sourced in Japan

TSKGel Alpha columns are high-performance liquid chromatography (HPLC) columns designed for the separation and analysis of various biomolecules, including proteins, peptides, and oligonucleotides. These columns utilize a proprietary packing material to provide efficient and reproducible separations.

Automatically generated - may contain errors

Lab products found in correlation

400 mhz spectrometer, by Bruker (4 mentions) Wyatt minidawn treos light scattering detector, by Wyatt Technology (4 mentions) Refractive index detector, by Wyatt Technology (1 mentions) Wyatt mini dawn treos light scattering, by Wyatt Technology (1 mentions)

7 protocols using tskgel alpha columns

1

Polymer Characterization by GPC and NMR

Check if the same lab product or an alternative is used in the 5 most similar protocols
PPS was characterized for molecular weight and polydispersity by gel permeation chromatography (GPC, Agilent Technologies, Santa Clara, CA, USA), and the chemical structure of the polymer was also analyzed by 1H NMR spectra recorded in CDCl3 (Brüker 400 MHz spectrometer). Molecular weight was measured using GPC with DMF + 0.1 M LiBr mobile phase at 60°C through three serial Tosoh Biosciences TSKGel Alpha columns (Tokyo, Japan). An Agilent refractive index (RI) detector and a Wyatt miniDAWN Treos multi-angle light scattering detector (Wyatt Technology Corp., Santa Barbara, CA, USA) were used to calculate absolute molecular weight based on dn/dc values experimentally determined using offline injections into the RI detector.
Poole K., Nelson C., Joshi R., Martin J., Gupta M., Haws S., Kavanaugh T., Skala M, & Duvall C. (2014). ROS-Responsive Microspheres for On Demand Antioxidant Therapy in a Model of Diabetic Peripheral Arterial Disease. Biomaterials, 41, 166-175.
+ Open protocol
+ Expand
2

Characterization of Polyphosphoester Structure

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
PPS was characterized for structure, molecular weight (Mn), and polydispersity (PDI) as described previously [25 (link),26 (link),30 (link)–32 (link)]. Gel permeation chromatography (GPC, Agilent Technologies, Santa Clara, CA, USA) was used to assess Mn and PDI of the polymer. PPS was dissolved in dimethylformamide (DMF) + 0.1 M LiBr and run through three serial Tosoh Biosciences TSKGel Alpha columns (Tokyo, Japan) at 60 °C. Offline injections into the Agilent refractive index (RI) detector were performed to determine the polymer dn/dc value. The dn/dc value was used to calculate absolute Mn using the RI detector and a Wyatt miniDAWN TREOS light scattering (LS) detector (Wyatt Technology Corp., Santa Barbara, CA).
DeJulius C.R., Bernardo-Colón A., Naguib S., Backstrom J.R., Kavanaugh T., Gupta M.K., Duvall C.L, & Rex T.S. (2020). Microsphere antioxidant and sustained erythropoietin-R76E release functions cooperate to reduce traumatic optic neuropathy. Journal of controlled release : official journal of the Controlled Release Society, 329, 762-773.
+ Open protocol
+ Expand
3

Characterization of Polyphenylsulfone Polymer

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PPS was characterized for structure, molecular weight, and polydispersity as described previously.36 (link),39 (link) The number-average molecular weight (Mn) and polydispersity (PDI) of PPS were assessed by gel permeation chromatography (GPC, Agilent Technologies, Santa Clara, CA, USA) using dimethylformamide (DMF) + 0.1 M lithium bromide mobile phase at 60 °C through three serial Tosoh Biosciences TSKGel Alpha columns (Tokyo, Japan). An Agilent refractive index (RI) and Wyatt miniDAWN TREOS light scattering (LS) detector (Wyatt Technology Corp., Santa Barabara, CA, USA) were used to calculate absolute molecular weight based on dn/dc values experimentally determined through a refractometer. The chemical structure of the PPS was confirmed by 1H nuclear magnetic resonance (NMR) recorded in CDCl3 with a Brüker 400 MHz spectrometer.
O’Grady K.P., Kavanaugh T.E., Cho H., Ye H., Gupta M.K., Madonna M.C., Lee J., O’Brien C.M., Skala M.C., Hasty K.A, & Duvall C.L. (2017). Drug-Free ROS Sponge Polymeric Microspheres Reduce Tissue Damage from Ischemic and Mechanical Injury. ACS biomaterials science & engineering, 4(4), 1251-1264.
+ Open protocol
+ Expand
4

Polymer Characterization by NMR and GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols
1H NMR spectra were collected for all polymers in CDCL3 on a Brüker 400 MHz spectrometer. Molecular weights (Mn), polydispersities (PI), and compositions were determined by either 1H NMR (Figure S3) or gel permeation chromatography (GPC) (Agilent Technologies, Santa Clara, Ca, USA) using dimethylformamide (DMF) + 0.1 M LiBr at 60 °C as the mobile phase through three serial Tosoh Biosciences TSKGel Alpha Columns (Tokyo, Japan). Serial dilutions (10 mg/mL – 0.25 mg/mL) were scanned on a digital refractometer to determine the refractive index increment (dn/dc) of polymers in order to calculate absolute molecular weights by GPC.
Uddin M.J., Werfel T.A., Crews B.C., Gupta M.K., Kavanaugh T.E., Kingsley P.J., Boyd K., Marnett L.J, & Duvall C.L. (2016). Fluorocoxib A Loaded Nanoparticles Enable Targeted Visualization of Cyclooxygenase-2 in Inflammation and Cancer. Biomaterials, 92, 71-80.
+ Open protocol
+ Expand
5

Polymer Characterization by NMR and GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols
1H NMR spectra of organic compounds and polymers were collected in CDCl3 with a Brüker 400 MHz spectrometer. GPC (Agilent Technologies, Santa Clara, CA, USA) was used to determine the Mn and PDI of polymers in dimethylformamide (DMF) +0.1 M LiBr mobile phase at 60 °C through three serial Tosoh Biosciences TSKGel Alpha columns (Tokyo, Japan). A Wyatt miniDAWN TREOS light scattering (LS) detector (Wyatt Technology Corp., Santa Barbara, CA, USA) and Agilent refractive index (RI) detector were used to calculate absolute Mn based on dn/dc values experimentally determined through off-line injection into the RI detector.
Gupta M.K., Lee S.H., Crowder S.W., Wang X., Hofmeister L.H., Nelson C.E., Bellan L.M., Duvall C.L, & Sung H.J. (2015). Oligoproline-derived nanocarrier for dual stimuli-responsive gene delivery. Journal of materials chemistry. B, 3(36), 7271-7280.
+ Open protocol
+ Expand
6

Polymer Characterization by NMR and GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols
1H NMR spectra of organic compounds and polymers were collected in CDCl3 with a Brüker 400 MHz spectrometer. GPC (Agilent Technologies, Santa Clara, CA, USA) was used to determine the Mn and PDI of polymers in dimethylformamide (DMF) +0.1 M LiBr mobile phase at 60 °C through three serial Tosoh Biosciences TSKGel Alpha columns (Tokyo, Japan). A Wyatt miniDAWN TREOS light scattering (LS) detector (Wyatt Technology Corp., Santa Barbara, CA, USA) and Agilent refractive index (RI) detector were used to calculate absolute Mn based on dn/dc values experimentally determined through off-line injection into the RI detector.
Gupta M.K., Lee S.H., Crowder S.W., Wang X., Hofmeister L.H., Nelson C.E., Bellan L.M., Duvall C.L, & Sung H.J. (2015). Oligoproline-derived nanocarrier for dual stimuli-responsive gene delivery. Journal of materials chemistry. B, 3(36), 7271-7280.
+ Open protocol
+ Expand
7

Polymer Characterization using GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polymer polydispersity and MW were characterized using GPC (Agilent Technologies). Polymers were dissolved at 10 mg/mL in DMF + 0.1 M LiBr mobile phase and run through three serial Tosoh Biosciences TSKGel Alpha columns at 60 °C. A Wyatt miniDAWN TREOS light scattering (Wyatt Technology Co.) and Agilent refractive index detector were used to calculate absolute number and weight-average MW based on dn/dc values determined on an offline refractometer (Anton Paar).
DeJulius C.R., Dollinger B.R., Kavanaugh T.E., Dailing E., Yu F., Gulati S., Miskalis A., Zhang C., Uddin J., Dikalov S, & Duvall C.L. (2021). Optimizing an Antioxidant TEMPO Copolymer for Reactive Oxygen Species Scavenging and Anti-Inflammatory Effects in Vivo. Bioconjugate chemistry, 32(5), 928-941.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!