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Exo1 a302 639a

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The EXO1 (A302-639A) is a laboratory instrument designed for the detection and quantification of exosomes. It utilizes specialized technology to isolate and analyze extracellular vesicles from various biological samples.

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Lab products found in correlation

Ripa lysis buffer, by New England Biolabs (1 mentions)

2 protocols using exo1 a302 639a

1

DNA Repair Protein Quantification

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AR (N-20), TOP1 (H-300), ATR (N-19), RAD50 (H-300), XRCC1 (H-300), BLM (H-300), DNA Ligase I (C-21), DNA Ligase IV (H-300), DNA POL β (C-21), DNA POL ε 3/CHRAC17 (N-15), p300 (C-20), SRC-1 (M-341) were from Santa Cruz Biotechnology. MRE11 (Ab397) and p-S1983-ATM (Ab2888) were obtained from Abcam. Ku80 (A302-627A) and EXO1 (A302-639A) were purchased from Bethyl Laboratories.
Puc J., Kozbial P., Li W., Tan Y., Liu Z., Suter T., Ohgi K.A., Zhang J., Aggarwal A.K, & Rosenfeld M.G. (2015). Ligand-dependent Enhancer Activation Regulated by Topoisomerase-I Activity. Cell, 160(3), 367-380.
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2

Immunoblot Analysis of DNA Repair Proteins

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Cell extracts were prepared using RIPA lysis buffer (NEB) with protease (Sigma) and phosphatase (Sigma) inhibitors. Immunoblots were performed using standard procedures. Samples containing proteins were separated using SDS PAGE 4–12% gradient gels (Invitrogen) and transferred onto nitrocellulose membranes. The membranes were incubated with primary and secondary antibodies. The primary antibodies were NUDT1 (NB100-109, Novus Biologicals), CHK2 (05–649, Millipore), POLM (C1, Santa Cruz), EXO1 (A302-639A, Bethyl Laboratories), FANCC (MABC524- clone 8F3, Millipore), POLE (GTX132100, GeneTex), Actin (A5060, SIGMA), NEIL1 (12145-1-AP, Proteintech), POLB (ab26343, Abcam), and MSH6 (D60G2, Cell Signalling). Catalogue numbers and working dilutions for antibodies are provided in Supplementary Table 1. Uncropped immunoblot images are shown in Supplementary Fig. 9.
Zou X., Owusu M., Harris R., Jackson S.P., Loizou J.I, & Nik-Zainal S. (2018). Validating the concept of mutational signatures with isogenic cell models. Nature Communications, 9, 1744.
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