Tryphostin ag 1478

W 123 (10 µM), JTE 013 (1–10 µM), CAY 10444 (10 µM), specific inhibitors of S1PR1, S1PR2, S1PR3 respectively, S1P (0.1–10 µM), and the EGFR inhibitor tryphostin AG-1478 (0.3–3 µM) were all obtained from Cayman Chemical (Ann Arbor, MI, USA). Helenalin (1 µM), inhibitor of NF-κB, pEGFR antibody (p-Tyr-845 SC-23420-R) and total EGFR antibody (SC-03) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). SR 11302 (1 µM), inhibitor of activator protein-1 (AP-1) and SEW 2871 (10 µm), agonist of S1PR1 were obtained from Tocris Bioscience (Bristol, UK). Dichlorodihydrofluorescein diacetate (DCFH-DA) (10 µM) and N-acetyl cysteine (NAC) (1 mM) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Luciferase reporter lysis buffer was obtained from Promega (Madison, WI, USA). GM6001 (25 µM), the broad-spectrum hydroxamic acid inhibitor of matrix metalloproteinases (MMPs) and TAPI-1 (10 µM), inhibitor or MMPs and tumor necrosis factor-α converting enzyme (TACE) were obtained from Calbiochem (La Jolla, CA). Fura-2 AM (10 µM), and pluronic F127 (0.02%) were obtained from Life Technologies (Carlsbad, Ca).

Collagenase type IV from Clostridium histolyticum was obtained from Sigma-Aldrich (St. Louis, MI, United States). EGFR inhibitor tryphostin AG1478 (0.3 μM) was obtained from Cayman Chemical (Ann-Arbor, MI, United States). BrdU flow kit was obtained from BD Biosciences (Franklin Lakes, NJ, United States). EGFR inhibitor afatinib (0.5 μM) was obtained from Santa Cruz (Santa Cruz, CA, United States). qPCR primers for mRNA targets and Lipofectamine 2000 were obtained from Thermo Fisher Scientific (Waltham, MA, United States). qPCR primers for miRNA targets and miR-210 mimic and inhibitors were obtained from Exiqon (Vedbaek, Denmark).