All digestive enzymes (α-amylase from porcine pancreas, pepsin from hog stomach, pancreatin from porcine pancreas, and bile extract porcine), magnesium hydroxide carbonate, calcium chloride dehydrate, magnesium chloride hexahydrate (99%), magnesium sulphate hexahydrate, sodium chloride, sodium bicarbonate and sodium phosphate were purchased from Sigma-Aldrich (St Louis, MO, USA). Potassium chloride was obtained from Panreac (Barcelona, Spain). Monopotassium phosphate was purchased from Acros Organics (New Jersey, USA). Butyl hydroxytoluene (BHT), hydrochloric acid and sodium hydroxide were acquired from Scharlau Chemie S.A. (Barcelona, Spain). Lycopene, γ-carotene, δ-carotene, β-carotene, lutein, phytofluene and phytoene were obtained from Carote-Nature (Ostermundigen, Switzerland).
β carotene
Manufactured by CaroteNature
Sourced in Switzerland
β-carotene is a naturally occurring organic compound that serves as a precursor to vitamin A. It is a common component found in various fruits and vegetables, particularly carrots, which give them their distinctive orange color. As a lab equipment product, β-carotene is used as a reference standard for analytical and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Lutein, by CaroteNature (12 mentions)
β cryptoxanthin, by CaroteNature (7 mentions)
Zeaxanthin, by CaroteNature (7 mentions)
α carotene, by CaroteNature (6 mentions)
Lycopene, by CaroteNature (4 mentions)
Violaxanthin, by CaroteNature (4 mentions)
Acetic acid, by Merck Group (3 mentions)
Phytoene, by CaroteNature (2 mentions)
Phytofluene, by CaroteNature (2 mentions)
γ carotene, by CaroteNature (2 mentions)
δ carotene, by CaroteNature (2 mentions)
Trolox, by Merck Group (2 mentions)
Neoxanthin, by CaroteNature (2 mentions)
Antheraxanthin, by CaroteNature (2 mentions)
Capsanthin, by CaroteNature (2 mentions)
Capsorubin, by CaroteNature (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Pepsin, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
17 protocols using β carotene
1
Comprehensive Gastrointestinal Enzyme Analysis
2
Carotenoid Quantification in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The carotenoid standards were lutein, zeaxantin, violaxantin, and β-carotene (CaroteNature, Münsingen, Switzerland). Gallic acid (842649), pepsin (P7000; ≥250 U/mg), pancreatin (P7545; 8xUSP), invertase (I4504; ≥300 U/mg), amyloglucosidase (A7095; ≥260 U/mL), Folin–Ciocalteu phenol reagent (109001), nitric acid 60% ultrapure (101518), and chemicals, at analytical or LiChrosolv grade, were purchased from Merck KGaA (Darmstadt, Germany).
3
Sourcing Biochemical Reagents for Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol, n-hexane, isopropanol, trichloromethane and HPLC grade dichloromethane, mEthanol, acetonitrile and water were purchased from Carlo Erba reagents (Val de Reuil, France). β-Carotene (HPLC purity > 96%) was from Carotenature GmbH (Münsingen, Switzerland). Retinol, retinyl palmitate, tocol, triolein, phospholipids, NaCl, sodium citrate, Tris hydroxide, bovine serum albumin and protease inhibitor cocktail were from Sigma Aldrich (Saint-Quentin Fallavier, France). Sevoflurane was from Baxter (Lublin, Poland), TRIzol reagent was from Euromedex (Souffelweyersheim, France). Dithiothreitol was from Thermo Fischer Scientific (Les Ulis, France). Phosphate buffered saline (PBS) was from life Technologies (Illkirch, France).
4
Characterization of Antioxidant Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbon dioxide (Industrial grade) was obtained from Praxair S.A. (Guadalajara, Mexico) and ethanol from Reactivos Guadalajara (Guadalajara, Mexico). The α-tocopherol, thiamine, riboflavin, niacin, pyridoxine, ammonium acetate, acetic acid, fatty acid methyl esters mix (Supelco 37 component FAME Mix), Folin–Ciocalteu reagent, sodium carbonate, DPPH, sodium acetate, TPTZ, ferric chloride hexahydrate, trolox, and ABTS were obtained from Sigma-Aldrich (Toluca, Mexico). Lutein and β-carotene (analytical grade) were purchased from CaroteNature (Lupsingen, Switzerland). Methanol, acetone, hexane, sulfuric acid and water (HPLC grade) were acquired from Tedia (Monterrey, Mexico). Helium and nitrogen were purchased from Infra S.A. (Monterrey, Mexico). All reagents were ACS grade, unless otherwise stated.
5
Carotenoid Standards Acquisition and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Violaxanthin, neoxanthin, antheraxanthin, lutein, zeaxanthin, phytoene, β-cryptoxanthin, phytofluene, α-carotene, β-carotene, ζ-carotene, δ-carotene, γ-carotene, neurosporene and lycopene were purchased from CaroteNature (Lupsingen, Switzerland). Methanol was purchased from Fisher Scientific (Waltham, MA, USA). Tert-methyl butyl ether (MTBE), chloroform and dichloromethane were purchased from Avantor Performance Materials (Panoli, Gujrat, India), hexane from Sigma chemical Co. (St. Louis, MO, USA) and ethanol from Hayman Ltd. (Essex, USA). All reagents were HPLC grade or higher. A Millipore Milli-Q water purification system was used to obtain high purity of water.
6
Quantification of Lipids and Carotenoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total lipid content was quantified gravimetrically following extraction with Folch reagent (2:1 chloroform/methanol) as described previously [19 (link)]. Carotenoids were extracted with acetone and quantified by high performance liquid chromatography (HPLC) as described previously [20 (link)]. Carotenoid standards β-carotene, torulene, and torularhodin were obtained from carotenature, GmbH (Ostermundigen, Switzerland).
7
Quantification of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents were purchased from various suppliers as follows: Authentic standards of kuromanin (cyanidin 3-O-glucoside chloride), chlorogenic acid (3-caffeoylquinic acid), gentisic acid, rutin (quercetin 3-O-rutinoside) (Extrasynthèse, Genay, France); the standard for carotenoids (lutein, carotene, α-carotene, β-carotene and lycopene) were purchased from CaroteNature (Lupsingen, Switzerland); gallic acid, Folin-Ciocalteu's phenol reagent, Trolox [(S)-(-)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid], ABTS [2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)], fluorescein disodium, AAPH [2,2′-azobis (2-methyl-propionamide)], ascorbic acid, meta-phosphoric acid, DTT (dithiothreitol), BHT (butylated hydroxytoluene) as well as acetonitrile, ethanol, methanol, acetone, methyl tert-butyl ether, formic acid and acetic acid (all HPLC grade) (Sigma-Aldrich, St. Louis, MO, USA). In all experiments Milli-Q (Merck Millipore, Darmstadt, Germany) water was used.
8
Carotenoid Standards Acquisition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standards of lutein, zeaxanthin, β-cryptoxanthin, lycopene, β-carotene and α-carotene were acquired from CaroteNature (GmbH, Lupsingen, Switzerland). HPLC-grade solvents including acetonitrile, methanol and ethyl acetate were obtained from Merck (KGaA, Darmstadt, Germany).
9
Detailed Methodology for Phytochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, formic acid, isopropanol, n-hexane, ethanol, methyl tert-butyl ether, butylated hydroxytoluene (BHT), HPLC-grade acetonitrile, and acetone were purchased from Fisher (Pittsburgh, PA, USA), all of which were HPLC-grade. Whereas, an Elix water purification system (Millipore, Billerica, MA, USA) was utilized to prepare in-house deionized water. The reference standards lutein (>96 % purity) and β-carotene (>99 % purity) were acquired from Carote Nature (Lupsingen, Switzerland) and measured by HPLC–PDA. Chlorophyll a (>92 % purity) and chlorophyll b (>92 % purity) were obtained from Sigma-Aldrich (St. Louis, MO, USA), and also measured by HPLC–PDA. The standards of delphinidin-3-O-glucoside (≥95 % purity), cyanidin (≥98 % purity), cyanidian-3,5-O-diglucoside (≥97 % purity), delphinidin (≥98 % purity), and cyanidin 3-O-galactoside (≥98 % purity) were obtained from Extrasynthese (Genay, France). All standards were of HPLC grade.
10
Carotenoid Standards Synthesis and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
α-Carotene, β-carotene, β-cryptoxanthin, lutein, zeaxanthin, antheraxanthin, and lutein-5,6-epoxide were purchased from Carote Nature GmbH (Münsingen, Switzerland). Zeinoxanthin used for standard was synthesized by Wittig condensation of C25-apocarotenal derived from commercially available α-ionone, with previously prepared C15-phosphonium salt possessing 3-hyxroxy-β-end group [33 (link)]. 1H NMR data of synthetic zeinoxanthin were identical with those reported in Reference [34 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!