All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
Gm02037
Manufactured by American Type Culture Collection
GM02037 is a human lymphocyte cell line derived from a male individual. It is available for purchase through the American Type Culture Collection (ATCC) for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ccd 841 con, by American Type Culture Collection (2 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (2 mentions)
Ccd 18lu, by American Type Culture Collection (2 mentions)
Gm05565, by American Type Culture Collection (2 mentions)
Bebm bronchial epithelial cell growth basal medium, by Lonza (2 mentions)
Hiec6, by Thermo Fisher Scientific (2 mentions)
Hiec 6, by American Type Culture Collection (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Begm singlequots, by Lonza (2 mentions)
2 protocols using gm02037
1
Cell Line Culture Optimization and Maintenance
2
Cell Line Culture Optimization and Maintenance
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All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
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