Jurkat cells

293T cells (DSMZ), GP2-293 cells (Takara Bio), and 293FT cells (Invitrogen) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine-penicillin-streptomycin solution (PSG; Sigma) at 37°C in a 5% CO₂ atmosphere. Jurkat cells (DSMZ), B-LCL cells (RIKEN Cell Bank), and NALM6 cells (ATCC) were cultured in RPMI-1640 (Sigma) supplemented with 10% FBS and 1% PSG at 37°C in a 5% CO₂ atmosphere.

Human T cell leukemia cell line Jurkat cells (RIKEN Cell Bank, Tsukuba, Japan) were retrovirally transduced with MSCV2.2 retroviral vectors (Vector Builder, Chicago, IL) containing T2R38 with functional haplotype (PAV haplotype) or empty vector and an internal ribosome entry site (IRES)-Green Fluorescent Protein (GFP) reporter^{33 (link)}. We inserted an HSV tag into the C-terminus. Briefly, the virus was produced using Platinum-A (Plat-A) cells (Cell Biolabs Inc, San Diego, CA), a potent retrovirus packaging cell line based on the 293 T cell line. Jurkat cells were spin-infected for 2 h with retroviral supernatant and cultured for migration assays.