To detect DR5 cell surface expression, indicated cells treated with lestaurtinib for 24 hours were incubated with anti‐DR5‐FITC and anti‐mouse IgG antibodies (Abcam) for 30 minutes at room temperature. The stained cells were analysed by the CellQuest Software with the FACSCalibur flow cytometer.
Anti dr5 fitc and anti mouse igg antibodies
Manufactured by Abcam
Sourced in United Kingdom
Anti-DR5-FITC and anti-mouse IgG antibodies are laboratory reagents used in various immunological and cell biology applications. Anti-DR5-FITC is a fluorescently labeled antibody that specifically binds to the Death Receptor 5 (DR5) protein, while anti-mouse IgG antibodies are used to detect and quantify mouse immunoglobulin G (IgG) in samples.
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Lab products found in correlation
2 protocols using anti dr5 fitc and anti mouse igg antibodies
1
Quantifying DR5 Surface Expression
2
Evaluating DR5 Expression and Knockdown
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To detect DR5 cell surface expression, PC-3 (2.5 × 10 5 ) cells exposed to TRAIL (20 ng/ml) and/or Vitisin A (4 µM) for 24 h were incubated with anti-DR5-FITC and anti-mouse IgG antibodies (Abcam, United Kingdom) for 30 min at room temperature. The stained cells were analyzed using the CellQuest Software with the FACSCalibur flow cytometer.
DR5 small interfering RNA transfection PC-3 cells were transiently transfected with a validated scrambled control siRNA vector or a DR5 siRNA plasmid (Santa Cruz Biotechnology, Santa Cruz, CA) using Interferin™ transfection reagent (Polyplus Transfection Inc., New York, NY, USA). Briefly, the mixture of siRNA and Interferin transfection reagent was incubated for 10 min, added to each well containing cells (final siRNA concentration = 40 nM) and incubated at 37°C for 24 h before Vitisin A (4 µM) and TRAIL (20 ng/ml) treatment.
DR5 small interfering RNA transfection PC-3 cells were transiently transfected with a validated scrambled control siRNA vector or a DR5 siRNA plasmid (Santa Cruz Biotechnology, Santa Cruz, CA) using Interferin™ transfection reagent (Polyplus Transfection Inc., New York, NY, USA). Briefly, the mixture of siRNA and Interferin transfection reagent was incubated for 10 min, added to each well containing cells (final siRNA concentration = 40 nM) and incubated at 37°C for 24 h before Vitisin A (4 µM) and TRAIL (20 ng/ml) treatment.
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