Glucose oxidase antibody

Polyamide substrates with a pore size of 200 nm and a thickness of 60 µm were obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA). The linker molecule dithiobis succinimidyl propionate (DSP), dimethyl sulfoxide (DMSO), and 1X phosphate buffered saline (PBS) were procured from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Salt-free streptavidin from Streptomyces avidiini (≥13 units/mg protein), alcohol oxidase enzyme from Pichia pastoris (10–40 units/mg protein), glucose oxidase from Asperigillus niger (100,000–250,000 units/g), D-(+)-glucose, sodium L-lactate (~98% purity), absolute ethyl alcohol (≥99.5%), and sodium bicarbonate (≥99.7%) were procured from Sigma-Aldrich (St. Louis, MO, USA). NHS-biotin was purchased from Vector laboratories (Burlingame, CA, USA). Glucose oxidase antibody was purchased from Abcam (Cambridge, MA, USA). Lactate oxidase (80 U/mg) was purchased from Toyobo USA. Synthetic sweat was prepared from the recipe described in M.T. Mathew et al. [20 (link)]. The pH range was varied by varying the concentrations of the constituents. Single donor human sweat of pH~6 was purchased from Lee Biosolutions Inc. (Maryland Heights, MO, USA). No preservatives were added to this product and it was stored at −20 °C. All alcohol, glucose, and lactate dilutions were made in synthetic sweat pH 6, 8, and in human sweat buffers.