Anti p mlc

The HUVEC line EA.Hy926 was obtained from American Type Culture Collection (ATCC, Manassas, VA). HUVECs were cultured with complete culture medium (RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicilin–streptomycin, 1% glutamine) and maintained at 37°C with 5% CO₂. Trypsin-EDTA was used to passage cells. All above agents were obtained from Gibco (Grand Island, New York, USA).
DTX was purchased from Xieli Pharmaceutical (Chengdu, China). CCK-8 kit and Annexin V-FITC Apoptosis Detection kit were purchased from Dojindo Laboratories (Shanghai, China) and KeyGEN Biotechnology (Nanjing, China), respectively. Anti-proliferative cell nuclear antigen (anti-PCNA) and anti-p53 were obtained from Proteintech Group (USA). Anti-β-actin, anti-GAPDH, anti-PECAM-1 and anti-integrin β1 were purchased from Santa Cruz Biotechnology (California, USA). Anti-VE-cadherin, anti-FAK, anti-pFAK, anti-Rho, anti-ROCK, anti-MLC2 and anti-pMLC were purchased from Abcam (Cambridge, UK). Anti-p190 RhoGEF was purchased from Bioss (Beijing, China). Y-27632 was purchased from Selleck chemicals (USA). HRP-conjugated and FITC/PE-conjugated anti-mouse/rabbit/goat secondary antibodies were obtained from Biosynthesis Biotechnology (Beijing, China).