Chemicals (Analytical grade, Sigma-Aldrich) were used without further purification.
Precaution: At elevated temperature, perchlorates are explosive in nature.
Analytical grade
Manufactured by Merck Group
Sourced in Germany, United States, China
Analytical grade laboratory equipment is designed for precise and accurate scientific measurements and analyses. These high-quality products are manufactured to meet rigorous standards, ensuring consistent performance and reliable results. They are commonly used in various research, quality control, and analytical applications across diverse industries.
Automatically generated - may contain errors
Lab products found in correlation
Azm dihydrate, by Merck Group (1 mentions)
6890n gc ms system, by Agilent Technologies (1 mentions)
Analytical standards, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Hp 5ms, by Agilent Technologies (1 mentions)
Methacholine, by Merck Group (1 mentions)
Hplc grade, by Mallinckrodt (1 mentions)
Mini protean 2 apparatus, by Bio-Rad (1 mentions)
1 adamantanecarboxylic acid, by Merck Group (1 mentions)
Mpd bm 3.5 apparatus, by Sanyo (1 mentions)
183 chns analyzer, by Leco (1 mentions)
300 mhz nmr spectrometer, by Bruker (1 mentions)
Nahco3, by Merck Group (1 mentions)
Zn no3 2 6h2o, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
18 protocols using analytical grade
1
Perchlorates: Explosive Handling Protocol
2
Synthesis and Characterization of AZM Dihydrate
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AZM dihydrate (C38H72N2O12·2H2O, the structure is provided in Scheme 1 ) (Analytical grade) from Sigma Aldrich (Darmstadt, Germany), and research-grade acetonitrile from Merck (Darmstadt, Germany), were purchased and used without further purification. The AC precursor, AF, was obtained from the Anzali wetland (Anzali City, Iran).
3
GC-MS Analysis of Ajowan Essential Oils
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A total of 6 µL of the ajowan EOs and 594 µL of analytical-grade n-hexane (Sigma-Aldrich, Milan, Italy) were injected in split-mode (split ratio 1:50) into an Agilent 6890 N GC-MS system, endowed with a 5973 N single quadrupole detector and an autosampler 7863. As a stationary phase, a 5% phenyl-methylpolysiloxane coated capillary column (Agilent HP-5MS, 30 m length, 0.25 mm internal diameter, 0.1 μm film thickness) was used. The temperature program was set as follows: 5 min isothermal at 60 °C, then ramp at 4 °C/min to 22 °C and, finally, ramp at 11 °C/min until 280 °C, maintained for 15 min. The flow rate of the carrier gas (helium 99.5%) was 1 mL/min and both the injector and detector were at 280 °C. The mass spectra were achieved between 29.0 uma and 400.0 uma in a full scan through the electron impact mode (EI, 70 eV). The major chemical constituents of the T. ammi EOs, namely p-cymene, γ-terpinene, and thymol, as well as carvacrol, were identified using analytical standards (Sigma-Aldrich, Milan, Italy), whereas the other components were identified by combining the temperature-programmed retention indices (RIs) and the mass spectra (MS), as detailed in our previous study [59 (link)].
4
Analytical Grade Chemical Procurement
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All chemicals were analytical grade or higher grade than analytical grade, purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise stated.
5
Curcumin extraction and dosage protocol
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An alcoholic extract was obtained from commercial turmeric (Curcuma Kosher, Lot 09076, AMBE Phytoextracts PVT LTD, Delhi, India). The concentration of CUR in the extract was determined by UV spectrophotometry at 230 nm, and the molecular identity was determined by the infrared spectrum compared to a CUR standard (analytical grade, Sigma Chemical Co., St. Louis, MO, USA). The food pellets were impregnated with the alcoholic extract, and then the alcohol was eliminated by evaporation at 60 °C for 4 h in darkness. The homogeneous distribution of CUR in the food pellets was corroborated by UV-spectrophotometry. The pellets containing 200 ppm of CUR (0.16 mg/g food) were dispensed as described by Nery-Flores, Mendoza-Magaña, Ramírez-Herrera, Ramírez-Vázquez, Romero-Prado, Cortez-Álvarez and Ramírez-Mendoza [37 (link)]. Consequently, each rat received approximately a dose of 5.6 mg/kg/day.
6
Enzyme Activity Measurement Protocol
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Research Article equation as expressed in nmol/mg, nmol/mg, and U/mg, respectively. The values of mean±standard deviation (SD) were obtained. The analytical grade from Sigma-Aldrich (Germany) was used for all measurements.
7
Purification of Solvents for Antimicrobial Analysis
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Solvents used for extraction (n-hexane, ethylacetate and methanol) were redistilled before use. Analytical grade (Sigma-Aldrich) of dimethyl sulphoxide (DMSO) was used as dissolving and diluting solvent for antimicrobial analysis.
8
BALB/c Mice Allergy Experiment
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BALB/c mice (specific pathogen-free grade, aged 5-6 weeks, weight 16-20 g) were purchased from the Animal Center of Guangdong Province (Guangzhou, China) and kept in a specific pathogen-free grade breeding room. This study was carried out strictly in accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Shenzhen University. Silkworm pupae were donated by the Sericulture and Agro-Processing Research Institute, Guangdong Academy of Agricultural Sciences (Guangzhou, China). Reagents and chemicals were analytical grade (Sigma, St Louis, MO, USA) and solvents were HPLC grade (Mallinckrodt, Lane Cove West, NSW, Australia). SDS/ PAGE/western blotting was performed by using a Mini Protean II apparatus (BioRad, Hercules, CA, USA) with 12 or 15% gels and a Tris/Tricine buffer system. Two dimensional PAGE was performed by using Immobiline IPG strips (13 cm) with a pH range of 3-10 (GE Healthcare Bio-Science, Piscataway, NJ, USA). Methacholine was purchased from Sigma-Aldrich (St Louis, MO, USA) and biotin-labeled goat anti-mouse IgE from Zymed Laboratories (San Francisco, CA, USA).
9
Quantifying Antioxidant Activities of Plant Extracts
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The antioxidant activities of POE, GA and TA were determined quantitatively using a DPPH RSA. The method was carried out using the protocol reported by Pontillas and Lapetaje (2019) . A 150-ppm stock solution of DPPH (Analytical Grade, Sigma-Aldrich Chemie GmbH, Germany) in methanol (ACS, Scharlau, Spain) was prepared. A 1000 ppm stock solution of POE, GA and TA was prepared and serially diluted to 10, 50, 100, 250 and 500 ppm. DPPH solution (2.3 mL) in methanol was added to the test samples, and the final volume was made up to 7.0 mL using methanol. After the incubation for 30 min in the dark, the absorbance of the test samples and the reference standardascorbic acid (AA) (Analytical Grade, Scharlau, Spain) was measured at 517 nm ( max ) against the blank (methanol) using the UV-Vis spectrophotometer. The percent (%) DPPH RSA for test sample concentration was calculated using Equation 3.
where A0 is the absorbance of the negative control; A1 is the absorbance in the presence of the sample.
where A0 is the absorbance of the negative control; A1 is the absorbance in the presence of the sample.
10
Utilization of Household SWCO for Biofuel
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Regeants used for the experimental work, were analytical grade purchased from Sigma-Aldrich (Sigma-Aldrich Trading Co., Shanghai, China). The renewable nutrient substrate was SWCO obtained from households (Cluj-Napoca, Romania). SWCO was used by itself as a carbon source for microbial growth, and it was also used as a lipidic substrate for transesteri cation reaction to obtain CG fraction.
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