We evaluated IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α production in coculture supernatants (days 1, 3 and 5), using Cytometric Bead Array (CBA Th1/Th2) (BD Company, USA), the FASCanto II (BD Company,USA), the BD FACS DIVATM program and the BD CBA Software (BD Company,USA). Concentration was calculated using the standard curves (FCAP Array Software BD Company,USA). Detection limits: 5 to 5000 pg/ml. For samples showing concentrations below the lower detection limit, we considered the value just below this limit to quantify cytokine production change in cocultures.
Cytometric bead array cba th1 th2
Manufactured by BD
Sourced in United States
The Cytometric Bead Array (CBA Th1/Th2) is a multiplex assay for the simultaneous quantification of multiple cytokines in a single sample. It utilizes fluorescent-coded beads coated with capture antibodies specific for different cytokines. This allows for the detection and measurement of multiple cytokines in a small sample volume.
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Lab products found in correlation
2 protocols using cytometric bead array cba th1 th2
1
Cytokine Production Quantification
2
Cytokine Profiling in Toxoplasma Infection
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Serum was obtained from infected or uninfected Mif−/− and WT mice during the acute phase of infection with T. gondii (1, 2, 3, 5, and 7 days after infection). The whole blood was centrifuged for 10 min at 2000 ×g at 4°C. MIF levels were determined by ELISA according to the manufacturer's guidelines (Neobiolab). Serum IFN-γ, IL-6, IL-12p70, IL-4, and IL-10 were measured using a Bio-Plex Pro Assay (BIO-RAD) or Cytometric Bead Array (CBA Th1/Th2, BD Biosciences) according to the manufacturer's protocols.
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