The largest database of trusted experimental protocols

Fibronectin coated transwell filters

Manufactured by Corning
Sourced in United States

Fibronectin-coated transwell filters are a type of lab equipment used for cell migration and invasion assays. These filters feature a porous membrane coated with the extracellular matrix protein fibronectin, which facilitates cell attachment and movement. The filters are designed to be used in a transwell system, allowing for the study of cellular behavior and responses.

Automatically generated - may contain errors

3 protocols using fibronectin coated transwell filters

1

Endothelial Permeability Assay in Brain Microvascular Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human brain microvascular ECs were obtained from Neuromics (Minneapolis, MN, USA; catalog number: #HEC02). Cells were cultured in a standard humidified atmosphere (37 °C) containing 5% CO2, as we described [58 (link)]. In some experiments, cells were treated with metformin (0.1, 0.5, and 1 mM), adding glucose at 5 or 25 mM, or mannitol 20 mM as osmotic control, for 48 h.
The endothelial permeability assay was performed as we described and validated [58 (link),59 (link)], using fibronectin-coated transwell filters (Corning Inc., Corning, NY, USA).
Levels of mitochondrial and cellular reactive oxygen species (ROS) were assessed using MitoSOX and CM-H2DCFDA dyes, respectively, as we previously described [60 (link),61 (link)].
Cell death was evaluated using a Caspase-Glo® 3/7 assay (Promega, Madison, WI, USA; catalog number: #G6321), as we described [62 (link)]. All reagents were from Millipore-Sigma (Burlington, MA, USA), unless otherwise stated. All experiments were conducted at least in triplicate by blinded investigators.
+ Open protocol
+ Expand
2

Assessing Endothelial Permeability in hBMECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human brain microvascular endothelial cells (hBMECs, passages 3–6) were cultured as we previously described7 (link), 49 (link). In some experiments, cells were treated with empagliflozin (1 μM), adding glucose at 5 or 30 mM, for 48 h. The endothelial permeability assay was performed using fibronectin-coated trans-well filters (Corning Inc., Corning, NY, USA), as we previously reported7 (link), 49 (link), 50 (link).
+ Open protocol
+ Expand
3

Transendothelial Migration Assay for PMNs and T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze transendothelial migration of PMNs or T cells across HUVEC monolayers, HUVECs were grown to confluence on fibronectin-coated transwell filters (5-µm pore size; Corning) and stimulated with 5 nM TNF 15 h before the assay and treated either with 5 µM AKB-9778 or vehicle. For the assay, 2.5 × 105 human PMNs or T cells were allowed to transmigrate for 30 min toward the chemokine IL-8 (for PMNs) or SDF-1α (for T cells). Transmigrated leukocytes were counted using a CASY Cell Counter TT+ (Roche).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!