Isolated human monocytes were labelled with APC-conjugated mouse monoclonal anti-CD14 and PE-conjugated mouse monoclonal anti-CD16 or isotype controls (all eBioscience, Hatfield, UK) according to manufacturer’s protocols. Murine blood, peritoneal lavage or air-pouch lavage cells were labelled with PE-conjugated rat monoclonal anti-Ly6G or anti-Gr1, PE-Cy5-conjugated rat monoclonal anti-CD115 and APC-conjugated rat monoclonal anti-F4/80, or isotype controls (all eBioscience, UK), all according to manufacturer’s protocols. In all cases, 20,000 events were acquired using a FACSCalibur flow cytometer (Becton Dickinson), and analysed using FlowJo analysis software (Version 9.6.3, Treestar Inc, Stanford, CA). The human and murine gating strategies for cellular analysis are shown in supplemental figures 2A and 2B , respectively. Actin polymerisation was assessed through binding of AF488-labelled phalloidin (Invitrogen, UK) according to manufacturer’s protocols.
Pe conjugated rat monoclonal anti ly6g or anti gr1
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
PE-conjugated rat monoclonal anti-Ly6G or anti-Gr1 is a laboratory reagent used for the detection and identification of Ly6G or Gr1-positive cells in flow cytometry applications. It is a PE-labeled antibody that binds specifically to the Ly6G or Gr1 antigen expressed on the surface of certain cell types.
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Lab products found in correlation
2 protocols using pe conjugated rat monoclonal anti ly6g or anti gr1
1
Multicolor Flow Cytometry Analysis of Leukocyte Populations
2
Characterizing Immune Cell Populations
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Isolated human monocytes were labeled with allophycocyanin-conjugated mouse monoclonal anti-CD14 and PE-conjugated mouse monoclonal anti-CD16 or isotype controls (all from eBioscience, Hatfield, U.K.), according to manufacturer’s protocols. Murine blood, peritoneal lavage, or air-pouch lavage cells were labeled with PE-conjugated rat monoclonal anti-Ly6G or anti-Gr1, PE-Cy5–conjugated rat monoclonal anti-CD115, and allophycocyanin-conjugated rat monoclonal anti-F4/80, or isotype controls (all from eBioscience), all according to manufacturer’s protocols. In all cases, 20,000 events were acquired using a FACSCalibur flow cytometer (BD Biosciences) and analyzed using FlowJo analysis software (Version 9.6.3; Tree Star, Stanford, CA). The human and murine gating strategies for cellular analysis are shown in Supplemental Fig. 2A and 2B , respectively. Actin polymerization was assessed through binding of AF488-labeled phalloidin (Invitrogen), according to manufacturer’s protocols.
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