Concentrations of human TGF-β (Elabscience, Wuhan, China, Cat#E-EL-0162c), TNF-α (Elabscience, Cat#E-EL-H0109c) and IL-6 (Elabscience, Cat#E-EL-H6156) secreted from the irradiated cells were measured with ELISA kits, in accordance with the instructions of the manufacturer. Briefly, cell culture media were collected and centrifuged at 1000 g (20 min, 4 °C) to remove cell debris. The supernatants were collected for ELISA assays.
E el 0162c
Manufactured by Elabscience
Sourced in China
The E-EL-0162c is a laboratory centrifuge designed for the separation of cellular components, proteins, and other biomolecules. It features a brushless motor and a compact design, allowing for efficient and reliable operation.
Automatically generated - may contain errors
Lab products found in correlation
E el r0012c, by Elabscience (2 mentions)
E el h0109c, by Elabscience (1 mentions)
E el h6156, by Elabscience (1 mentions)
Multiskan mk3, by Thermo Fisher Scientific (1 mentions)
E msel m0003, by Elabscience (1 mentions)
E msel m0001, by Elabscience (1 mentions)
E msel m0002, by Elabscience (1 mentions)
E el m3001, by Elabscience (1 mentions)
E el r0016c, by Elabscience (1 mentions)
E el r0015c, by Elabscience (1 mentions)
E el r2856c, by Elabscience (1 mentions)
E el r0567c, by Elabscience (1 mentions)
E el r3021, by Elabscience (1 mentions)
E el r0009c, by Elabscience (1 mentions)
5 protocols using e el 0162c
1
Quantification of Immune Cytokines
2
Quantification of Inflammatory Cytokines in Mice
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The concentrations of IL-1 α (cat. no. E-EL-M3059, Elabscience), IL-1 β (cat. no. E-MSEL-M0003, Elabscience), IL-6 (cat. no. E-MSEL-M0001, Elabscience), MMP-3 (cat. no. E-EL-M0626c, Elabscience), TNF-α (cat. no. E-MSEL-M0002, Elabscience), TGF- β1 (cat. no. E-EL-0162c, Elabscience), and MCP-1 (E-EL-M3001, Elabscience) in mice were determined using an ELISA kit. All tests were performed according to the manufacturer's instructions. Absorbance was measured at 450 nm using an automatic enzyme labeling instrument (Thermo Scientific, Multiskan MK3).
3
Cytokine Profiling in Hippocampus
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After modeling (9 days after surgery), the whole blood and hippocampus tissues were harvested. After 2 h of standing, the whole blood was centrifuged for 3 min at 9,000 rpm. The serum was then collected to analyze the expression of cytokines. The hippocampus tissues were homogenized, and the levels of IL-1β (Elabscience, E-EL-R0012c), IL-6 (Elabscience, E-EL-R0015c), IL-12 (Elabscience, E-EL-R0064c), tumor necrosis factor (TNF)-α (Elabscience, E-EL-R2856c), IL-18 (Elabscience, E-EL-R0567c), colony-stimulating factor (CSF)-1 (Elabscience, E-EL-R0601c), IL-4 (Elabscience, E-EL-R0014c), IL-10 (Elabscience, E-EL-R0016c), transforming growth factor (TGF)-β (Elabscience, E-EL-0162c), and IL-13 (Elabscience, E-EL-R0563c) were determined according to the manufacturers’ instructions.
4
Skin Tissue Cytokine Expression Analysis
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We evaluated the expression of transforming growth factor-β1 (TGF-β1) (E-EL-0162c, Elabscience), matrix metallopeptidase 9 (MMP9) (E-EL-R3021, Elabscience), interleukin-1β (IL-1β) (E-EL-R0012c, Elabscience), toll-like receptor 2 (TLR2) (E-EL-R0907c, Elabscience), Interferon Gamma (IFN-γ) (E-EL-R0009c, Elabscience) in rat skin tissue by ELISA. Briefly, skin tissue was homogenized by a tissue homogenizer in RIPA lysis buffer on ice. After centrifugation at 12,000 rpm for 15 min at 4°C, supernatants were collected and prepared for the ELISA experiment. The samples were incubated on microtiter plates for 1.5 h, followed by biotinylated secondary antibodies for 1 h. Next, the slides were incubated with a Horseradish Peroxidase (HRP) conjugate peroxidase working solution for 30 min. Finally, tetramethylbenzidine (TMB) substrate solution was added, and after 15 min incubation in the dark, stop solution was then added to the microtiter plates. Absorbance was measured at 450 nm using a microplate reader.
5
CTGF and TGF-β Quantification
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The levels of CTGF and TGF-β were detected using CTGF ELISA kit (E-EL-M0340c; Elabscience, China) and TGF-β ELISA kit, respectively (E-EL-0162c; Elabscience), as described in the manufacturer’s instructions.
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