B6 cg tg cd4 tgfbr2 16flv j

dnTGFβRII (TG) mice on a C57BL/6 back-ground (B6.Cg-Tg(Cd4-TGFBR2)16Flv/J) [13 (link)] were initially derived from Jackson Laboratory. The maintenance and genetic monitoring of this colony has been previously reported [14 ]. CXCR3 knockout mice on a C57BL/6 background were previously established by gene targeting [16 (link)] and were kindly provided by Dr. Bao Lu (Harvard Medical School, Boston, MA, USA). dnTGFβRII CXCR3−/− (TGC3) mice were obtained by selectively backcrossing TG mice with CXCR3 knockout mice. 8–14 week old female TGC3 mice were used in all experiments. B6/Rag1−/− mice (Ly5.2) were obtained from Jackson Laboratory. 8–9 week old female Rag1−/− mice were used as recipients in our CD8+ T adoptive transfer model. All mice were housed in a specific pathogen-free and controlled environment (22°C, 55% humidity, and 12-h day/night rhythm) and care provided according to the regulations of animal care at University of Science and Technology of China (Hefei, Anhui, China).

dnTGFβRII (TG) mice with a C57BL/6 background (B6.Cg-Tg(Cd4-TGFBR2)16Flv/J) [21 (link)] were initially derived from stock from the from Jackson Laboratory (Bar Harbor, ME, USA). The maintenance and genetic monitoring of this colony has been previously reported [22 ]. TLR2 knockout mice also on a C57BL/6 background were kindly provided by Dr. Shao- Bo Su (Sun Yat-sen University, Guangzhou, Guangdong, China). dnTGFβRIITLR2−/− (TGT2) mice were obtained by selectively backcrossing dnTGFβRII mice with TLR2 knockout mice. Wide-type (WT) littermates were obtained by crossing dnTGFβRII mice with WT mice. All mice were housed in a specific pathogen-free and controlled environment (22 °C, 55% humidity, and 12-h day/night rhythm) and care was provided according to the regulations of animal care at the University of Science and Technology of China (Hefei, Anhui, China).