Antibodies recognizing TMEFF2 from cell lysates were purchased from Abcam (Cambridge, MA) and Sigma (St. Louis, MO). For TMEFF2 detection from mouse tissue lysates a SDIX, custom antibody was utilized (SDIX, Newark, DE). Other antibodies utilized in this study are: SV40 T-antigen (Abcam; Cambridge, MA), ITGAV, ITGB1 and ITGB3 (BD Biosciences, Franklin Lakes, NJ), ITGB3 (for mouse tissue lysates, Sigma, St. Louis, MO), ITGA5 (Millipore, Billerica, MA), ribosomal protein S6, β-actin, phospho-FAK, and FAK (Cell Signaling, Danvers, MA). Antibodies used for the immunofluorescence studies are listed in section 2.5. The U0126 MAPK inhibitor or the inactive analog U0124 were purchased from Sigma (St. Louis, MO). Bovine serum albumin (BSA) was from Sigma (St. Louis, MO).
Itgb1
Manufactured by BD
Sourced in United States
ITGB1 is a laboratory equipment product. It is designed to perform a core function related to its intended purpose. The details of its specific functionality and intended use are not available in this concise and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using itgb1
1
Antibody Detection of TMEFF2 Protein
2
Proteoglycan Analysis by Western Blot
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In brief, equivalent amounts of cell lysates (30 μg) were applied to 8 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and blotted with antibodies against CD44 (Bioss, BSM51065M), CHSY1 (OriGene, Cat: TA315190, Rockville, MD, USA), CHPF (Proteintech, Cat: 239531AP, Chicago, IL, USA), ITGB1 (BD bioscience, Cat: 610467, Franklin Lakes, NJ, USA) and actin (Santa Cruz, sc-47778). For some experiments, protein lysates were treated with 0.01 U of chondroitinase ABC (ChaseABC, Sigma-Aldrich, Burlington, MA, USA) or 0.1 μg of heparinase II and III (R&D SystemsTM, Minneapolis, MN, USA) for 2 h at 37 °C to remove the CS or HS chains on the proteoglycan.
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