30mL of venous blood from study volunteers was collected in blood collection tubes containing sodium heparin from BD Biosciences. Whole blood was layered on top of 15mL of Histopaque 1077 (Sigma-Aldrich) in 50mL Leucosep blood separation tubes (Greiner Bio-One) and spun at 1000g for 18min at RT. Plasma was collected and stored at −80°C. The PBMC layer was collected and washed 2x in sterile PBS before red blood cell lysis with ACK buffer (Quality Biologicals). PBMCs were used in assays either immediately or frozen down for use in the near term.
Leucosep blood separation tubes
Manufactured by Greiner
Leucosep blood separation tubes are designed for the isolation and separation of mononuclear cells from whole blood samples. The tubes contain a specialized density gradient medium that enables the separation of cellular components during centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using leucosep blood separation tubes
1
PBMC Isolation from Whole Blood
2
Isolation of Whole Blood Plasma
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30mL of venous blood from study volunteers was collected in blood collection tubes containing sodium heparin from BD Biosciences. Whole blood was layered on top of 15mL of Histopaque 1077 (Sigma-Aldrich) in 50mL Leucosep blood separation tubes (Greiner Bio-One) and spun at 1000g for 18min at RT. Plasma was collected and stored at −80°C until further use.
3
Isolation and Cryopreservation of PBMCs
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30mL of venous blood from study volunteers was collected in blood collection tubes containing sodium heparin from BD Biosciences. Whole blood was layered on top of 15mL of Histopaque 1077 (Sigma-Aldrich) in 50mL Leucosep blood separation tubes (Greiner Bio-One) and spun at 1000g for 18min at RT. Plasma was collected and stored at -80°C. The PBMC layer was collected and washed 2x in sterile PBS before red blood cell lysis with ACK buffer (Quality Biologicals). PBMCs were used in assays either immediately or frozen down for use in the near term, as freezing cells does not significantly affect antigen specific T cell reactivity (21 (link)).
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