Af488 conjugated donkey anti rabbit

Cells were fixed with paraformaldehyde (PFA) and treated with either BSA (extracellular staining) or BSA/Saponin to measure intracellular staining. The following antibodies were used to detect Zika virus: anti-Flavivirus 4g2 mouse IgG2a (NovusBio), anti-Flavivirus 4g2 monoclonal rabbit (Absolute Antibody), anti-Zika virus monoclonal Rabbit (Genetex).
All other antibodies were anti-human: DC-SIGN mouse IgG1 (AZN-D1), anti-langerin mouse IgG1 (10E2) both in house made, PE conjugated CD207 (langerin), APC conjugated CD1a (BD Biosciences), CD86-FITC (BD Pharmingen), CD80-PE (BD Pharmingen), CD83-APC (BD Pharmingen), DC-SIGN-FITC (R&D systems), CD3-APC/Fire750 (Biolegend), CD11c-APC (Biolegend), PEcy7-HLA-DR (BD Pharmingen), APCcy7-CD14 (BD Biosciences), APCcy7-CD11c (Biolegend),APC-AXL (Thermofisher, PE-MerTK (Thermofisher) and anti-Tyro3 (Thermofisher). For secondary detection the following antibodies were used: AF488-conjugated goat anti-mouse IgG2a (Invitrogen), AF647-conjugated donkey anti-rabbit (Thermofisher), FITC-conjugated goat-anti-mouse IgM (Invitrogen), AF488-conjugated donkey anti-rabbit (Thermofisher).
Flow cytometric analyses were performed on a BD FACS Canto II (BD Biosciences) and data was analysed using FlowJo V10 software (TreeStar).

Firefly luciferase (FLuc) mRNA (5moU) (L-7202), Cre mRNA (5moU) (L-7211) and Cas9 mRNA (5moU) (L-7206) were purchased from TriLink BioTechnologies. DNA barcode (b-DNA) design parameters followed our previous report^{38 (link)}. A full list of b-DNA sequences can be found in Supplementary Data 2. Scrambled negative control sgRNA (Cat#A35526, Thermo Fisher Scientific) and VEGFR2 sgRNA (Synthego) were used as received. All oligonucleotides were purchased from Integrated DNA Technologies and were purified through standard desalting procedures. Luciferase 1000 Assay System (Ref. E4550) and CellTiter-Glo Luminescent Cell Viability Assay Kit (Ref. G7572) were purchased from Promega Corporation. Anti-mouse CD31 antibody (AF488, Cat#102514, Clone#MEC13.3), CD45 antibody (Brilliant Violet 421, Cat#103134, Clone#30-F11), and EpCAM antibody (AF647, Cat#118212, Clone#G8.8) were purchased from BioLegend. Draq7 (Cat#424001, BioLegend) and 7AAD (Cat#A1310, Thermo Fisher Scientific) were used for live/dead staining. Tumor-bearing lung slices were stained with primary antibodies including goat anti-mouse/rat CD31 (Cat#AF3628, R&D Systems) and rabbit anti-mouse GFP (Cat#ab183734, abcam), followed by staining with secondary antibodies including AF488-conjugated donkey anti-rabbit (Cat#A-21206, Thermo Fisher Scientific) and AF555-conjugated donkey anti-goat (Cat#A32816, Thermo Fisher Scientific).