Log In Sign up
The largest database of trusted experimental protocols

Ab224232

Manufactured by Abcam
Visit Supplier

Ab224232 is a laboratory equipment product. It is designed to perform a specific core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

M085129 2, by Agilent Technologies (2 mentions) Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) A10037, by Thermo Fisher Scientific (1 mentions) A11034, by Thermo Fisher Scientific (1 mentions) D9542, by Merck Group (1 mentions) Ab14041, by Abcam (1 mentions)

2 protocols using ab224232

1

PDE5 and α-SMA Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence analyses were carried out as previously described.65 (link) Primary antibodies: rabbit polyclonal anti-PDE5 (ab224232, abcam) and mouse monoclonal anti-α-SMA (M085129-2, Dako), Secondary antibodies: Alexa Fluor 568 donkey anti mouse IgG and Alexa Fluor 488 goat anti rabbit IgG (A10037 and A11034 respectively, Invitrogen). Cell nuclei were counterstained with 1 μg/ml DAPI (4’,6’-diamidino-2-phenylindole, D9542, Sigma-Aldrich).
Sharpe B.P., Hayden A., Manousopoulou A., Cowie A., Walker R.C., Harrington J., Izadi F., Breininger S.P., Gibson J., Pickering O., Jaynes E., Kyle E., Saunders J.H., Parsons S.L., Ritchie A.A., Clarke P.A., Collier P., Mongan N.P., Bates D.O., Yacqub-Usman K., Garbis S.D., Walters Z., Rose-Zerilli M., Grabowska A.M, & Underwood T.J. (2022). Phosphodiesterase type 5 inhibitors enhance chemotherapy in preclinical models of esophageal adenocarcinoma by targeting cancer-associated fibroblasts. Cell Reports Medicine, 3(6), 100541.
+ Open protocol
+ Expand
2

Immunohistochemical Staining of PDE5, α-SMA, and Periostin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Optimization and staining of cohort on full face sections using rabbit polyclonal anti-PDE5 (1:250 dilution with Dako FLEX TRS High pH retrieval; ab224232, abcam), mouse monoclonal anti-α-SMA (1:200 dilution with Dako FLEX TRS High pH retrieval; M085129-2, Dako) and rabbit polyclonal anti-Periostin (1:1000 dilution with Dako FLEX TRS Low pH retrieval; ab14041, abcam) was performed on a Dako link automated staining machine according to the manufacturer’s instructions.
Sharpe B.P., Hayden A., Manousopoulou A., Cowie A., Walker R.C., Harrington J., Izadi F., Breininger S.P., Gibson J., Pickering O., Jaynes E., Kyle E., Saunders J.H., Parsons S.L., Ritchie A.A., Clarke P.A., Collier P., Mongan N.P., Bates D.O., Yacqub-Usman K., Garbis S.D., Walters Z., Rose-Zerilli M., Grabowska A.M, & Underwood T.J. (2022). Phosphodiesterase type 5 inhibitors enhance chemotherapy in preclinical models of esophageal adenocarcinoma by targeting cancer-associated fibroblasts. Cell Reports Medicine, 3(6), 100541.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!