Pdgf bb
Manufactured by R&D Systems
Sourced in United States, United Kingdom, China, Germany
PDGF-BB is a recombinant human Platelet-Derived Growth Factor-BB protein. PDGF-BB is a dimeric disulfide-linked protein that belongs to the platelet-derived growth factor family. It is a key regulator of cell growth, cell proliferation, and chemotaxis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (20 mentions)
Vascular endothelial growth factor (vegf), by R&D Systems (11 mentions)
Tgf β1, by R&D Systems (10 mentions)
Tgf β, by R&D Systems (10 mentions)
Dmem f12, by Thermo Fisher Scientific (10 mentions)
Hepatocyte growth factor (hgf), by R&D Systems (8 mentions)
Pdgf ab, by R&D Systems (8 mentions)
Interleukin 6 (il 6), by R&D Systems (7 mentions)
Pdgf aa, by R&D Systems (7 mentions)
Fibronectin, by Merck Group (5 mentions)
Vegf a, by R&D Systems (5 mentions)
Penicillin, by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Bmp 2, by R&D Systems (4 mentions)
Axiovert, by Zeiss (4 mentions)
Tnf α, by R&D Systems (4 mentions)
Basic fibroblast growth factor (bfgf), by R&D Systems (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Il 1β, by R&D Systems (3 mentions)
171 protocols using pdgf bb
1
Serum Biomarker Quantification Protocol
2
Generating Long-term DEHP-exposed HSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HSC-T6 cells [25 (link)] were cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY, USA) with 1% heat-inactivated foetal bovine serum (HyClone, Logan, UT, USA), penicillin (100 U/mL), streptomycin (100 μg/mL), non-essential amino acids (0.1 mM), and L-glutamine (2 mM) in a humidified incubator with 5% CO2. To generate long-term, low-dose, DEHP-exposed HSCs, HSC-T6 cells were exposed to 50 and 100 µM DEHP (Alfa Aesar, USA), and the medium containing DEHP was renewed every 2–3 days. After 3.5 months, long-term, low-dose, DEHP-exposed HSCs were obtained. Cell morphology was recorded by using an Olympus CKX41 microscope (Tokyo, Japan). To study the effect of long-term exposure to low-dose DEHP on PDGF-BB-induced cell proliferation, cells were seeded in 6-well plates (2.5 × 105), and then were treated with 10 ng/mL PDGF-BB (R&D Systems, Minneapolis, MN, USA) for different amounts of time. The cells were then subjected to the following proliferation and Western blot analyses.
3
Stem Cell Differentiation and Bone Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Collagenase P (Roche, IN, USA), hyaluronidase (Sigma Aldrich, St Louis, MO, USA), Ad-GFP (Vector Biolabs, Cat No. 1060), Ad-Cre (Vector Biolabs, Cat No. 1045), PDGF-BB (R&D Systems, Minneapolis, MN, USA), recombinant human BMP2 (PeproTech, Rocky Hill, NJ, USA), TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), ImProm II kit (Promega, USA), TaqMan Universal Mastermix (Thermo Fisher Scientific, UK), SYBR green-based assay (Thermo Fsher, Waltham, MA, USA; Life Technologies, Grand Island, NY, USA), Protease Inhibitor cocktail (Roche), tamoxifen (Sigma Aldrich, St. Louis, MO, USA), corn oil (Acros Organics, Belgium), Cryomatrix (Thermo Scientific, Waltham, MA, USA), Leukocyte acid phosphatase kit (Sigma Aldrich, St. Louis, MO, USA), BMP2 (Medtronic, MN, USA) and PDGF BB (R&D, 520-BB-050/CF), methyl methacrylate bone cement (Orthodontic Resin, Dentisply Caulk Inc. Milford, DE, USA). Antibodies used are listed in Supplementary Table 1 .
4
PDGF-BB Stimulation of Human Airway Smooth Muscle Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human airway smooth muscle cells (HASMCs) were supported by American Type Culture Collection. The cells were grown in a six‐well plate in dulbecco's modified eagle medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin‐streptomycin at 37°C with 5% CO2. Cells at passages 2–3 were used for following experiments.
For the PDGF‐BB induction group, HASMCs were first serum deprived for 24 h and then induced with 25 ng/mL PDGF‐BB (R&D Systems) for 24 h.36 Experiments were repeated three times independently.
For the PDGF‐BB induction group, HASMCs were first serum deprived for 24 h and then induced with 25 ng/mL PDGF‐BB (R&D Systems) for 24 h.
5
Rat Aortic VSMC Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rat aortic VSMCs (rVSMCs) were purchased from Cell Applications Inc. (San Diego, CA, USA) and cultured in smooth muscle cell basal medium (SmBM, Cell Applications) with 5% FCS and the appropriate supplementation. Cells at passages 3–5 were seeded onto 10 cm culture dishes (5 × 103 cells/cm2) and incubated at 37 °C in 5% CO2 for 72 h. After a wash with PBS, the rVSMCs were incubated for 24 h in serum- and supplement-free SmBM to arrest cell growth. Subsequently, the medium was changed to fresh α-MEM with 1%FCS and PDGF-BB (20 ng/ml; R&D systems, MN, USA) or to the MSC-CdM with 1%FCS and PDGF-BB. The cells were harvested 24 h after the stimulation, and the cell counts were measured as described previously [18] (link). The cell number ratio was defined as cell number divided by the number at baseline. The cells harvested on day 3 after the stimulation were used for western blotting and real time RT-PCR analyses.
6
Renal HRMC Culture and Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary renal HRMCs were purchased from ScienCell (San Diego, CA). HRMCs from the third to sixth passages were maintained in MC Medium (MsCM; ScienCell) according to the manufacturer's instructions. For experiments, the cells were grown to confluence, growth‐arrested in reduced serum (0.5% FBS) for 24 hours and split into different experimental groups.
For treatment with different concentrations of glucose, the cells were incubated in MsCM containing either 5 mmol/L glucose (normal glucose) or 30 mmol/L glucose (high glucose, HG) for 48 hours at 37°C. Cells incubated under identical experimental conditions with 5 mmol/L glucose and 25 mmol/L mannitol instead of 30 mmol/L glucose were used as osmotic controls. For treatment with PDGF‐BB, the cells were incubated in MsCM containing 20 ng/mL PDGF‐BB (R&D Systems, Minneapolis, MN) for 48 hours at 37°C.
For treatment with different concentrations of glucose, the cells were incubated in MsCM containing either 5 mmol/L glucose (normal glucose) or 30 mmol/L glucose (high glucose, HG) for 48 hours at 37°C. Cells incubated under identical experimental conditions with 5 mmol/L glucose and 25 mmol/L mannitol instead of 30 mmol/L glucose were used as osmotic controls. For treatment with PDGF‐BB, the cells were incubated in MsCM containing 20 ng/mL PDGF‐BB (R&D Systems, Minneapolis, MN) for 48 hours at 37°C.
7
Generation of Induced Stromal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Generation of iSTCs was performed according to our previous study with a slight modification33 (link) as summarized in Figure S3 B. In brief, hiPSCs were plated on iMatrix-511 coated dishes in hiPSC medium supplemented with ROCK inhibitor Y-27632 (10 μM) before differentiation. We used DMEM/F12 (Gibco) containing 1x StemFit for Differentiation (AS401) (Ajinomoto Co., Inc) and 1% Glutamax as a base medium. For mesoderm induction stage, hiPSCs were cultured in the base medium supplemented with CHIR99021 (8 μM, Tocris Bioscience) and BMP4 (25 ng/mL). Medium was changed every day until day 3 of induction. On day 4, medium was changed to stromal cell induction medium consisting of the base medium supplemented with Activin A (2 ng/mL, PeproTech Inc.) and PDGF-BB (10 ng/mL, R&D Systems). From day 8, the medium was changed to stromal cell maturation medium consisting of the base medium supplemented with PDGF-BB (10 ng/mL). After the stromal cell induction stage, the medium was changed every 2 days.
8
PDGF-BB Stimulation in RPASMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPASMCs were generous gifts from Dr. Zeng Yan, and cultured as previously reported [45 (link)]. Cultured primary RPASMCs (≤4 passage) were starved for 12 h (h) and then treated with PDGF-BB (30 ng/ml, R&D system, Minneapolis, MN) for different time (0, 2, 4, 6, 12, 24 h). miRNA mimics (20nM) were transfected with K2 transfection reagent (Biontex, Planegg, Germany) in RPASMCs. HEK-293a, a cell line derived from human embryonic kidney, was purchased from American Type Culture Collection (Manassas, USA). The transfection of miRNA mimic and DNA plasmids into HEK-293 cells were performed with polyethylenimine (PEI, Geneups, Shenzhen, China).
9
Quantifying Endothelial Secretome Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the quantification of cytokines, chemokines and angiogenic factors secreted by the endothelial cells, Enzyme-Linked Immuno Sorbent Assays (ELISA) were performed using commercially available ELISA antibodies. All reagents were used in accordance to the manufacturer’s specifications. uPA, PDGF-BB, PAI-1, Angiopoietin-2, VEGF, HGF, Il-6, CXCL1, CXCL10, CXCL12, CCL2, CCL5, CCL20, CCL27 (all R&D Systems, Abingdon, UK) and CXCL8 (Sanquin, Amsterdam, The Netherlands). ELISA results are expressed as amount of angiogenic factor/cytokine/chemokine in ng/mL.
10
PDGF-BB-Induced ASMC Proliferation Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this experimental study, human ASMCs were available from the Cell Bank of the Chinese
Academy of Sciences (Shanghai, China). Subsequently, cells were cultivated in DMEM
(Corning, Manassas, VA, USA) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin,
and 100 μg/mL streptomycin (Thermo Fisher Scientific, MA, USA) in 5% CO2 at
37˚C. When cells reached 90% confluence, subculture was carried out. ASMCs were treated
with different concentration of PDGF-BB (0, 1, 10, 20, 40, 60 mg/mL, R&D Systems,
Minneapolis, MN, USA) for different times (1-48 hours) to construct the in
vitro model of asthma.
MiR-140-3p mimics (miR-140-3p), mimic negative control
(miR-NC), miR-140-3p inhibitors (miR-140-3p-in),
inhibitor negative control (miR-in), pcDNA3.0-HMGB1(HMGB1) and empty vector pcDNA3.0 were available from RiboBio
(Guangzhou, China). Subsequently, ASMCs were transferred into a 24-well cell plate at
3×105 cells/well, and cultured at 37˚C in 5% CO2 for 24 hours, and
then the cells were transfected by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Academy of Sciences (Shanghai, China). Subsequently, cells were cultivated in DMEM
(Corning, Manassas, VA, USA) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin,
and 100 μg/mL streptomycin (Thermo Fisher Scientific, MA, USA) in 5% CO2 at
37˚C. When cells reached 90% confluence, subculture was carried out. ASMCs were treated
with different concentration of PDGF-BB (0, 1, 10, 20, 40, 60 mg/mL, R&D Systems,
Minneapolis, MN, USA) for different times (1-48 hours) to construct the in
vitro model of asthma.
MiR-140-3p mimics (miR-140-3p), mimic negative control
(miR-NC), miR-140-3p inhibitors (miR-140-3p-in),
inhibitor negative control (miR-in), pcDNA3.0-HMGB1(HMGB1) and empty vector pcDNA3.0 were available from RiboBio
(Guangzhou, China). Subsequently, ASMCs were transferred into a 24-well cell plate at
3×105 cells/well, and cultured at 37˚C in 5% CO2 for 24 hours, and
then the cells were transfected by Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!