50 μl of cell lysate were mixed with CA II reaction buffer containing 12.5 mM TRIS pH 7.5, 75 mM NaCl and 2 mM 4-nitrophenylacetate (50 μl, Sigma). Absorbance was monitored at 400 nm for 5 min. Conversion to 4-nitrophenol was calculated from the slope of the absorbance plot using a calibration line from different dilutions of 1 mM 4-nitrophenol (Sigma).
4 nitrophenol
Manufactured by Merck Group
Sourced in United States, Germany, India, Spain, Belgium
4-nitrophenol is a chemical compound used in various laboratory applications. It is a pale yellow crystalline solid with a molar mass of 139.11 g/mol. The compound is soluble in organic solvents and has diverse analytical and synthetic applications in chemical research and development.
Automatically generated - may contain errors
Lab products found in correlation
Sodium borohydride, by Merck Group (26 mentions)
Sodium hydroxide (naoh), by Merck Group (9 mentions)
Silver nitrate, by Merck Group (7 mentions)
Hydrochloric acid (hcl), by Merck Group (6 mentions)
Phenol, by Merck Group (6 mentions)
4 nitroaniline, by Merck Group (5 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (5 mentions)
2 nitrophenol, by Merck Group (4 mentions)
Ethanol, by Merck Group (4 mentions)
Nabh4, by Merck Group (4 mentions)
Hydrogen peroxide, by Merck Group (4 mentions)
Sulfuric acid, by Merck Group (4 mentions)
Ascorbic acid, by Merck Group (4 mentions)
4 aminophenol, by Merck Group (4 mentions)
Sodium citrate, by Merck Group (3 mentions)
Sodium alginate, by Merck Group (3 mentions)
4 chlorophenol, by Merck Group (3 mentions)
Triton x 100, by Merck Group (3 mentions)
Nitrobenzene, by Merck Group (3 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
111 protocols using 4 nitrophenol
1
Carbonic Anhydrase II Activity Assay
2
Asphaltene Extraction from Turkish Crude Oil
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Potassium hydroxide (KOH, ≥ 85%), silver nitrate (AgNO3, ≥99.5%), ammonium hydroxide (NH4OH, 30-33% NH3), hydrochloric acid (HCl(aq), 36.38%), 2,4-dinitrophenol (DNP, ≥98%) and n-heptane (≥99% HPLC) were provided by Sigma Aldrich, and 4-nitrophenol (PNP) and 2,4,6-trinitrophenol (TNP) were provided by Merck. Asphaltene was extracted from Turkish crude oil supplied from the West Raman region located in Batman. In the experiments, deionized water, produced by the Nuve ND 4 water distiller with storage tank, was used.
3
Synthesis of Metal Nanoparticles
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Sodium alginate (MW = 250000 Da) and carboxymethyl cellulose (MW = 90.000 g/mol) were purchased from Sigma Aldrich as sources of natural polymers, FeCl3‧6H2O, FeCl3‧6H2O and FeCl2·4H2O (Merck Co.) were used as precursors of Fe3O4 NPs, CuSO4·5H2O (Merck Co.) was used as a precursor of Cu NPs, and 4-nitrophenol (Merck Co.), sodium borohydride (NaBH4), and sodium citrate (Merck Co.) were used. All of the materials were used as received without further purification.
4
Electrochemical Characterization of Phenolic Compounds
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All chemicals
were of analytical grade and
used without further purification. Pyrocatechol, hydroquinone, and
2-aminophenol were purchased from SDFCL (India). 4-Nitrophenol was
purchased from Merck. DA was obtained from Sigma. Na2SO4 was purchased from Koch-Light Laboratory Chemicals Ltd. (England).
KI, NiCl2·6H2O, and CoCl2 were
obtained from BDH Laboratory Chemicals Ltd. (England). Na2HPO4 and NaH2PO4 were purchased
from ADWIC Laboratory Chemicals (Egypt). Phosphate-buffered saline
supporting electrolyte (PBS, pH = 7.0) was prepared from NaH2PO4 (0.1 mol L–1) and Na2HPO4 (0.1 mol L–1).
were of analytical grade and
used without further purification. Pyrocatechol, hydroquinone, and
2-aminophenol were purchased from SDFCL (India). 4-Nitrophenol was
purchased from Merck. DA was obtained from Sigma. Na2SO4 was purchased from Koch-Light Laboratory Chemicals Ltd. (England).
KI, NiCl2·6H2O, and CoCl2 were
obtained from BDH Laboratory Chemicals Ltd. (England). Na2HPO4 and NaH2PO4 were purchased
from ADWIC Laboratory Chemicals (Egypt). Phosphate-buffered saline
supporting electrolyte (PBS, pH = 7.0) was prepared from NaH2PO4 (0.1 mol L–1) and Na2HPO4 (0.1 mol L–1).
5
Biosynthesis of Metal Nanoparticles
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Silver nitrate
(Merck),l -ascorbic acid (Merck), l -alanine (Hi-Media),
sodium borohydride (Merck), methylene blue (Thomas Baker), rhodamine
B (Thomas Baker), 4-nitrophenol (SRL), benzoquinone (Merck), ammonium
oxalate (Merck), isopropanol (Merck), gentamycin (Sigma-Aldrich),
Luria broth (Hi-Media), and Luria agar (Hi-Media). The bacterial strains
of E. coli (MTCC 405) and S. aureus (MTCC 3160) were procured from the Microbial
Type Culture Collection (MTCC), Institute of Microbial Technology
(Chandigarh, India). Analytical grade chemicals were obtained and
used without further purification. Washing and cleaning of glassware
were done carefully with nitric acid followed by distilled water before
use. Deionized water was utilized throughout the experiments.
(Merck),
sodium borohydride (Merck), methylene blue (Thomas Baker), rhodamine
B (Thomas Baker), 4-nitrophenol (SRL), benzoquinone (Merck), ammonium
oxalate (Merck), isopropanol (Merck), gentamycin (Sigma-Aldrich),
Luria broth (Hi-Media), and Luria agar (Hi-Media). The bacterial strains
of E. coli (MTCC 405) and S. aureus (MTCC 3160) were procured from the Microbial
Type Culture Collection (MTCC), Institute of Microbial Technology
(Chandigarh, India). Analytical grade chemicals were obtained and
used without further purification. Washing and cleaning of glassware
were done carefully with nitric acid followed by distilled water before
use. Deionized water was utilized throughout the experiments.
6
Synthesizing Novel Organic Compounds
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All reagents and solvents were of analytical grade (>95%) and commercially available. Phosphoric acid, purchased from Sigma Aldrich, is the only exception (85%). Potassium iodide, sodium phosphate dibasic, potassium nitrate, 5-hydroxyisophthalic acid, 5-aminoisophthalic acid, 3-hydroxybenzyl alcohol, and resorcinol were also purchased from Sigma Aldrich. Iodine resublimed, sodium dihydrogen phosphate dihydrate, and 4-nitrophenol were purchased from Merck. Tri-sodium phosphate dodecahydrate and 5-sulfosalicylic acid were obtained from BDH, while benzoic acid was obtained from Astrochimica. Water, formic acid, and acetonitrile used for UPLC-PDA-HR-MS analysis were MS-grade and were provided by CARLO ERBA Reagents.
7
Synthesis and Adsorption of Metal-Organic Composites
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Aerosil A-380 (Evonik Degussa GmbH, Essen, Germany), magnesium acetate tetrahydrate, Mg(CH3COO)2·4H2O (ACS reagent, ≥98%, Merck, KGaA, Darmstadt, Germany), manganese(II) acetate tetrahydrate, Mn(CH3COO)2·4H2O, (99.99%, Sigma-Aldrich, KGaA, Darmstadt, Germany), nickel(II) acetate tetrahydrate, Ni(CH3COO)2·4H2O (98%, Sigma-Aldrich), copper(II) acetate hydrate, Cu(CH3COO)2·H2O, zinc acetate dehydrate, Zn(CH3COO)2·2H2O (ACS reagent, ≥98%, Sigma-Aldrich), resorcinol (99.9%, Chimlaborreativ, Brovary, Ukraine) and a 37% aqueous solution of formaldehyde ((stabilized with about 10% methanol) for synthesis, Sigma-Aldrich) were used in the synthesis of composites. Double-distilled water was used as the solvent. In the adsorption experiment, 4-nitrophenol and 4-chlorophenol, delivered by Merck (Darmstadt, Germany), were used as adsorbates.
8
Synthesis and Characterization of Titanium Dioxide Nanoparticles
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All chemicals with analytical grade were used without further purification. Titanium (IV) isopropoxide (C12H28O4Ti, ≥ 98%), potassium bromate (KBrO3, ≥ 98%), potassium iodide (KI, ≥ 98%), benzoquinone (C6H4O2, ≥ 98%), isopropyl alcohol (C3H8O, ≥ 98%), urea (CH4N2O, ≥ 98%), and absolute ethanol (C2H5OH, ≥ 99%) were purchased from Acros-Organics. Glacial acetic acid (CH3COOH, ≥ 98%), 1, 5- Di-phenylcarbazide (C13H14N4O, ≥ 98%), Triton X-100 (C16H26O2, ≥ 99%), terpineol (C10H18O, ≥ 98%), and cellulose ethane (C34H66O24, ≥ 99%) were all obtained from Sigma-Aldrich. Methylene blue (C16H18ClN3S, ≥ 98%), sodium hydroxide (NaOH, ≥ 99%), and potassium dichromate (K2Cr2O7, 98%) were supplied from Fluka, while Rhodamine B (C28H30N2O3, ≥ 98%), sodium sulfate (Na2SO4, ≥ 99%), and 4-nitrophenol (C6H5NO3, ≥ 98%) were obtained from Merck. Hydrochloric acid (HCl, 37%) and absolute acetone (C3H6O, ≥ 99%) were purchased from Riedel-de Haen. Potassium hydroxide (KOH, ≥ 99%), Nafion perfluorinated (C7HF13O5S.C2F4, ≥ 98%), and potassium chloride (KCl, ≥ 99%) were obtained from Chem-Lab. The reference electrode Ag/AgCl with electrolyte concentration CKCl = 3 mol/L was supplied from Metrohm. Finally, microscope slides were purchased from Fisher Scientific, while deionized (DI) water was used throughout.
9
Grape Fruit Disinfection and Characterization
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Grape (Red Globe) were purchased from Tulufan, Xinjiang, China. All fruits were free from mechanical damage or infection. The surface of the selected fruits was disinfected with 75% ethanol for 5 min, washed twice with sterile water, and air-dried.
Low molecular weight chitosan (85% deacetylated) and agar powder (bacteriological grade) were procured from Sigma-Aldrich (St. Louis, MO, USA). Silver nitrate (AgNO3, 99.8%; Energy Chemical, Shanghai, China), acetic acid (glacial, 99 100%; Sinopharm, Beijing, China), sodium citrate (95%; Sinopharm), and sodium hydroxide (NaOH, Sinopharm) 4-nitrophenol (95%; Merck, Darmstadt, Germany) were used as received without further purification. All other chemicals used in this study were of analytical grade.
Low molecular weight chitosan (85% deacetylated) and agar powder (bacteriological grade) were procured from Sigma-Aldrich (St. Louis, MO, USA). Silver nitrate (AgNO3, 99.8%; Energy Chemical, Shanghai, China), acetic acid (glacial, 99 100%; Sinopharm, Beijing, China), sodium citrate (95%; Sinopharm), and sodium hydroxide (NaOH, Sinopharm) 4-nitrophenol (95%; Merck, Darmstadt, Germany) were used as received without further purification. All other chemicals used in this study were of analytical grade.
10
Quantification of Nitroaromatic Compounds
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Riboflavin (Merck), BOC-l -cystine (Sigma), ethyl methyl ketone (Merck), ethanol (Merck), p-toluene sulfonic acid (Merck) were used. The analyte picric acid (PA) was provided by Sigma. 3,5-Dinitrosalicylic acid (DNSA), dinitro benzene (DNB), 2-nitrophenol (2NP), nitrobenzene (NB), 4-nitrophenol (4NP), nitromethane (NM), nitroethane (NE), benzoic acid (BA) and chloroform were obtained from Merck.
CAUTION! The nitroaromatic compounds need to be handled very carefully. picric acid must be kept in purified water. For humans, the safety limit of exposure to PA is 0.1 mg m−3.
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