Szx7 stereomicroscope

Manufactured by Olympus

Sourced in Japan

The SZX7 stereomicroscope is a versatile optical instrument designed for a wide range of research and industrial applications. It provides high-quality magnification and illumination for detailed observation and analysis of specimens. The SZX7 features a zoom range, allowing users to adjust the magnification level as needed. It is equipped with advanced optical components to deliver clear, high-resolution images. The instrument is suitable for a variety of sample types and can be adapted to meet specific requirements.

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Stereomicroscope (279 citations)

64 protocols using szx7 stereomicroscope

Detailed Spider Morphology Protocol

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Spiders were fixed and preserved in 80% ethanol. Specimens were examined with an Olympus SZX7 stereomicroscope; details were studied with an Olympus CX41 compound microscope. Female epigynes and male palps were examined and illustrated after dissection. Epigynes were removed and cleared in warm lactic acid before illustration. The image of the vulva was made after being embedded in Arabic gum. Photos were made with a Cannon EOS70D digital camera mounted on an Olympus CX41 compound microscope. The digital images were taken and assembled using the Helicon focus 6.80 software package.
All measurements were obtained using an Olympus SZX7 stereomicroscope and are given in millimetres. Eye diameters were measured at the widest point. The total body length does not include the chelicerae or spinnerets. Leg lengths are given as total length (femur, patella, tibia + metatarsus, tarsus). The terminology used in the text and figure legends follows Yu and Li (2019) (link).
All specimens are deposited in the Museum of Guizhou Education University, Guiyang, Guizhou, China (MGEU, curator Hao Yu).

Zhang J., Yu H, & Chen J. (2020). First description of the female of Clubiona milingae Barrion-Dupo, Barrion & Heong, 2013 (Araneae, Clubionidae). Biodiversity Data Journal, 8, e51789.

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Quantifying Aortic Atherosclerotic Lesions

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The heart and the aorta were placed in ice-cold oxygenated PSS buffer. Fat-and connective-tissue were carefully removed using micro scissors under an Olympus SZX7 stereomicroscope. The trimmed aorta was cut longitudinally from the arch to the iliac artery, and then, flatted and mounted between an objective glass and a cover slide, avoiding any overlapping tissue. Digital images of the intimal surface were obtained using an Olympus SZX7 stereomicroscope and Olympus Color View I camera. The level of aorta of atherosclerotic lesions was quantified as the percentage of plaque covering the intima surface area of the aorta and was evaluated and quantified by a person blinded to the exposure groups. Data analysis was carried out using ImageJ software.

Christophersen D.V., Møller P., Thomsen M.B., Lykkesfeldt J., Loft S., Wallin H., Vogel U, & Jacobsen N.R. (2021). Accelerated atherosclerosis caused by serum amyloid A response in lungs of ApoE^-/- mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3).

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Quantification of Aortic Atherosclerosis

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The heart and the aorta were placed in ice-cold oxygenated PSS buffer where fat-and connectivetissue were carefully removed using micro scissors under an Olympus SZX7 stereomicroscope. The trimmed aorta was cut longitudinally from the arch to the iliac artery, and then flattened and mounted between an objective glass and a cover slide, avoiding any overlapping tissue. Digital images of the intimal surface were obtained using an Olympus SZX7 stereomicroscope and Olympus Color View I camera. Atherosclerotic plaque burden was quantified as the percentage of lesions covering the intima surface area of the aorta and quantified by a person blinded to the exposure groups. Data analysis was carried out using ImageJ software.

Christophersen D.V., Jacobsen N.R., Andersen M.H., Connell S.P., Barfod K.K., Thomsen M.B., Miller M.R., Duffin R., Lykkesfeldt J., Vogel U., Wallin H., Loft S., Roursgaard M, & Møller P. (2016). Cardiovascular health effects of oral and pulmonary exposure to multi-walled carbon nanotubes in ApoE-deficient mice. Toxicology, 371.

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Microscopic Assessment of Beetle Pores

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Ten beetles of each sex, collected in traps during field bioassays in 2014, were examined using an Olympus SZX7 stereomicroscope to assess presence/absence of pores in males and females. Images were taken using a Canon EOS 70D camera attached to a light microscope digital SLR adapter on a Nikon SMZ1000 stereomicroscope equipped with a Plan Apo 1X WD70 objective lens (8–80x total magnification).
Specimens for histological sectioning were collected in live traps (described below), placed adjacent to the Adams Produce site (see below for details about field site). Specimens were submerged in ethanol-based hand sanitizer as a fixative. Fixation, sectioning, and imaging were contracted to Laudier Histology (New York, NY). Images were acquired from microscope slides with a Carl Zeiss AxioImager Z1 microscope, running ZEN Blue image processing software (Zeiss, Oberkochen, Germany).

Ray A.M., Francese J.A., Zou Y., Watson K., Crook D.J, & Millar J.G. (2019). Isolation and identification of a male-produced aggregation-sex pheromone for the velvet longhorned beetle, Trichoferus campestris. Scientific Reports, 9, 4459.

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Colony Formation and Cell Viability Assays

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For the colony formation assay, 1000 cells were plated in 6-well plates. The cells were cultured for 14 days and stained with 0.1% crystal violet. The cell colonies were photographed, and the number of colonies comprising more than 50 individual cells was counted using SZX7 stereo microscope (Olympus, Tokyo, Japan). For the cell viability assay, cells (2000 to 3000 cells/well) were dispensed in 100 μL culture medium in a 96-well plate, and incubated for the indicated time. EZ-Cytox cell viability kit (Daeil-Lab, Seoul, Korea) solution (10 μL) was mixed with the culture medium in each well of the plate. Samples were incubated for 1 h at 37 °C, and the absorbance of each sample at 450 nm was measured using a microplate reader (PerkinElmer, Waltham, MA, USA).

Lee K.H., Kim B.C., Jeong S.H., Jeong C.W., Ku J.H., Kim H.H, & Kwak C. (2020). Histone Demethylase KDM7A Regulates Androgen Receptor Activity, and Its Chemical Inhibitor TC-E 5002 Overcomes Cisplatin-Resistance in Bladder Cancer Cells. International Journal of Molecular Sciences, 21(16), 5658.

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Dissection and Photography of Insect Genitalia

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Specimens of each species were dissected, and the genitalia placed in a drop of glycerol on glass slides. After photography, genitalia were transferred to a plastic mount pinned with the respective specimen. Habitus photographs were taken using an Axioskop 40 compound microscope with AxioCam HRc Rev. 3/3.3v (4164×3120). Photographs of genitalia were taken using an Olympus SZX7 stereomicroscope, and subsequently combined with Auto-Montage software. The SEM photograph was taken using a Phenom Prox scanning electronic microscope. Complete label data are provided for type specimens, exact label data in English being cited for the type material (data in Chinese are translated into English). All specimens used in this study are deposited in the collection of Sun Yat-sun University, Guangzhou, China (

SYSU

Jia F, & Tang Y.D. (2018). A faunistic study of genus Chasmogenus Sharp, 1882 of China (Coleoptera, Hydrophilidae). ZooKeys.

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Whole-mount in situ hybridization of Pax3 in E15 mouse embryo gut

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The E15 embryo guts were dissected and fixed in 4% PFA overnight in 4°C. The whole mount RNA in situ hybridization was performed as described previously [58 (link)] with anti-Pax3 probe hybridization at 68°C. The images were captured with Olympus DP70 camera attached to the Olympus SZX7 stereo microscope.

Kim H., Langohr I.M., Faisal M., McNulty M., Thorn C, & Kim J. (2018). Ablation of Ezh2 in neural crest cells leads to aberrant enteric nervous system development in mice. PLoS ONE, 13(8), e0203391.

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Microscopic Examination of Insect Specimens

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Specimens were observed and measured using an Olympus SZX7 stereomicroscope. The microscope was equipped with 10× eyepieces, a DF PL 2×_-4 objective (16–112× magnification) and a calibrated ocular micrometer. Genitalia and tarsal claws were relaxed in hot water and dissected. Dissections were placed in glycerine on glass slides for observation. For additional observations and images of the prosternal process, aedeagi and tarsal claws, selected specimens were cleared in a warm 10% potassium hydroxide (KOH) solution and periodically checked multiple times an hour. Once desired elimination of soft tissue was achieved, specimens were thoroughly rinsed in DI (deionized) water. In some cases, DNA voucher specimens were used for observation and imaging of structures as the lysing process also dissolves soft tissue, effectively clearing the specimen and negating the need to damage additional specimens.

Baca S.M, & Short A.E. (2021). Review of the New World Notomicrus Sharp (Coleoptera, Noteridae) I: Circumscription of species groups and review of the josiahi group with description of a new species from Brazil. ZooKeys, 1025, 177-201.

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Histological Analysis of Rat Cardiac Remodeling

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Rat HWI and LVMI were analyzed 12 weeks after SFI treatment using the following equations: HWI = heart weight/body weight, and LVMI = left ventricular weight/ body weight.
Pathological changes in the myocardial tissue were observed by hematoxylin-eosin (HE) staining. Five to six rats randomly selected from each of the three groups and anesthetized with pentobarbital sodium (30 mg/kg IP) 12 weeks after SFI treatment. The hearts were removed, washed with NS for 10 min, and fixed in neutral-buffered 4% formalin for 48 h. Paraffin-embedded samples were sliced to 4-μm sections and HE stained^{6 (link)}. Photographs were obtained using an Olympus SZX7 stereomicroscope and inverted microscope (Olympus Co., Tokyo, Japan).

Mao Z.J., Zhang Q.L., Shang J., Gao T., Yuan W.J, & Qin L.P. (2018). Shenfu Injection attenuates rat myocardial hypertrophy by up-regulating miR-19a-3p expression. Scientific Reports, 8, 4660.

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Microscopic Mold Assessment on Wood

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Assessment of visible mold was made by looking vertically down at a fixed distance of 30 cm from the sample lit from four directions. Photographs were taken under the same circumstances. Microscopic mold on wood samples was assessed by an experienced microscopist at Lund University using a mold index scale (MI) ranging from 0 to 4 [44 (link)]. It was performed using an Olympus SZX7 stereo microscope (Shinjuku, Tokyo, Japan) at 40× magnification and low-angle light to detect fungal structures: hyaline (transparent) or dematiaceous (brown-colored) hyphae and conidiophores (spore-producing hyphae) [44 (link)]. The MI scale is as follows: 0 = no mold growth; 1 = initial growth, with one or a few hyphae and no conidiophores; 2 = sparse but clearly established growth, often conidiophores are beginning to develop; 3 = patchy, heavy growth with many well-developed conidiophores; and 4 = heavy growth over more or less the entire surface [44 (link)].

Lorentzen J.C., Ekberg O., Alm M., Björk F., Harderup L.E, & Johanson G. (2024). Mold Odor from Wood Treated with Chlorophenols despite Mold Growth That Can Only Be Seen Using a Microscope. Microorganisms, 12(2), 395.

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