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3 protocols using 1 2 3 trihydroxybenzene

1

Comprehensive Chemical Reagent Procurement

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Sodium chloride, methanol, ethanol, amino acid standards, acetonitrile, phenyl isothiocyanate, gallic acid, triethylamine, n-hexane, boron trifluoride-methanol, dichloromethane, formic acid, diethyl ether, petroleum ether, sodium hydroxide, potassium hydroxide, hydrochloric acid, and perchloric acid were purchased from ANPEL Laboratory Technologies (Shanghai) Inc. Vitamin B1/B2 and vitamin E were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), mixed standards of fatty acids, cyanidin 3-O-glucoside, anthranone, 1,2,3-trihydroxybenzene, benzoyl-dl-arginine p-nitroanilide hydrochloride, dimethyl sulfoxide, sulfosalicylic acid, trypsin, and phytic acid were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The purity of chemicals used for High-Performance Liquid Chromatography (HPLC) and Gas Chromatography Mass Spectroscopy (GCMS) complied with the requirement of relevant procedures. All other chemicals were of analytical grade.
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2

Antioxidant and Anti-Inflammatory Assay Protocol

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Diphenyl-1-picrylhydrazy (DPPH), tannic acid, L-ascorbic acid, Folin Ciocalteu’s phenol reagent, 2,2’-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS), butylated hydroxyanisole (BHA), dimethyl sulfoxide (DMSO), potassium persulfate (K2S2O8), trizma base, 1,2,3-trihydroxybenzene, 2,3-dihydroxyphenol, pyrogallic acid, MTT, xanthine oxidase, xanthine, elastase, N-succinyl-Ala-Ala-Ala-P-nitroanilide, collagenase from Clostridium histolyticum, and 4-phenylazobenzyloxy-carbonyl-Pro-Leu-Gly-Pro-D-Arg were purchased from Sigma-Aldrich (St. Louis, MO, USA). Monobasic sodium phosphate and dibasic sodium phosphate were purchased from Duksan Chemical (Ansan, Korea). RIPA buffer, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and a penicillin/streptomycin mixture (P/S) were obtained from Gibco BRL Co. (Grand Island, NY, USA). Detailed information for the reagents is presented in Supplementary Table S2.
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3

Characterization of Caulerpa lentillifera Polysaccharides

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Caulerpa lentillifera was supplied by Longlou seaweed farm, Wenchang, Hainan province, China. Monosaccharide standards (D-galactose (Gal), L-rhamnose (Rha), Larabinose (Ara), L-fucose (Fuc), D-glucose (Ghlc), D-xylose (Xyl), D-mannose (Man), and D-glucuronic acid (GlcA)), dextrans (MW: 196 kDa, 43.5 kDa, 9 .9 kDa, 1.46 kDa, and 106 Da), 5-methyl-2-phenyl-1,2-dihydropyrazol-3-one (PMP), bovine serum albumin (BSA), Coomassie brilliant blue G-250, 5,5-two methyl-1-pyridine-N-oxide (DMPO), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), and 1,2,3-Trihydroxybenzene were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
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