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Pe conjugated anti igd

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PE-conjugated anti-IgD is a laboratory reagent used for the identification and analysis of IgD-expressing cells. It consists of a phycoerythrin (PE) fluorescent dye conjugated to an antibody that binds specifically to the IgD protein. This product can be used in flow cytometry and other immunoassay applications to detect and quantify IgD-positive cells.

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Lab products found in correlation

Apc conjugated anti b220, by BD (2 mentions) Lsrfortessa, by BD (1 mentions) Percp conjugated anti cd45, by BioLegend (1 mentions) Fitc conjugated anti cd3, by BioLegend (1 mentions) Apc cy7 conjugated anti cd19, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Cellquest pro software, by BD (1 mentions) 7 amino actinomycin d staining, by BD (1 mentions) Brdu flow kit, by BD (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Fitc conjugated anti gl7, by BD (1 mentions) Pe conjugated np, by Biosearch Technologies (1 mentions) Pe cy7 conjugated anti cd95, by BD (1 mentions) Pe cy7 conjugated anti b220, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Anti cd138, by BD (1 mentions) Anti cd86, by BD (1 mentions) Anti cd95, by BD (1 mentions) Anti cd23, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-IgD (26 citations) IgD PE (18 citations) Anti-IgD-PE (12 citations)

4 protocols using pe conjugated anti igd

1

Isolation and Immunophenotyping of PBMCs

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PBMCs were isolated by centrifugation for 30min at 100g in 4°C. The whitish buffy coat between histopaque and medium was aspirated and was washed twice with 10 ml of sterile PBS. Frozen PBMC samples w thawed and were immunolabelled with following anti-human monoclonal antibodies: PerCP-conjugated anti-CD45 (Biolegend, San Diego, CA, USA), FITC-conjugated anti-CD3 (Biolegend, San Diego, CA, USA), APC-Cy7-conjugated anti-CD19 (BD Biosciences, San Jose, CA, USA), APC-conjugated anti-CD27 (Biolegend, San Diego, CA, USA) and PE-conjugated anti-IgD (BD Biosciences, San Jose, CA, USA). For memory B-cell transcriptome study using Nanostring assay, lymphocytes positive for CD19 and CD27 were sorted in 1.5 ml tubes. After 30 minutes of incubation in refrigerator at 4°C, samples were read by BD LSR Fortessa (BD Biosciences, San Jose, CA, USA).
Min Y.G., Park C., Kwon Y.N., Shin J.Y., Sung J.J, & Hong Y.H. (2019). B Cell Immunophenotyping and Transcriptional Profiles of Memory B Cells in Patients with Myasthenia Gravis. Experimental Neurobiology, 28(6), 720-726.
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2

Multicolor Flow Cytometry Analysis

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Allophycocyanin-conjugated anti-CD27 and anti-IgG, PerCP-cyanin 5.5-conjugated anti-CD19, and PE-conjugated anti-IgD and their conjugated isotype controls were all from BD Biosciences. Polyvalent goat IgG FITC-anti-IgM antibodies were from The Jackson Laboratory (Mississauga, Ontario, Canada). All stainings were performed using 1 μg of each Ab for 1 × 106 cells. Cells were fixed with 2% paraformaldehyde. Isotype-matched control Ab staining was >95% double-negative cells. Regions containing dead cells were delineated using 7-amino-actinomycin D staining, following manufacturer's instructions (BD Biosciences). Analyses were done by gating ≥10,000 cells with a FACSCalibur Flow cytometer and the CellQuest Pro software (BD Biosciences). Data were subsequently analyzed with FCS Express II software (De Novo Software, Thornhill, ON, Canada).
Dugas-Bourdages E., Néron S., Roy A., Darveau A, & Delage R. (2014). Persistent Polyclonal B Cell Lymphocytosis B Cells Can Be Activated through CD40-CD154 Interaction. Advances in Hematology, 2014, 854124.
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3

Multiparametric Flow Cytometry of Mouse B Cell Subsets

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Flow cytometry analysis of mouse NB and GCB was performed using the following fluorescent-labeled anti–mouse antibodies: APC-conjugated anti-B220 (BD Pharmingen), PE-Cy7-conjugated anti-CD95 (BD Pharmingen), FITC-conjugated anti-GL7 (BD Pharmingen) and PE-conjugated CXCR4 (eBioscience). Cell cycle analysis was performed using the BrdU Flow Kit (BD Pharmingen) and antigen-specific GCB (NP+GL7+CD95+B220+) were detected using PE-conjugated NP (biosearch Technologies, Inc.). Ex vivo stimulated B cells were stained with PE-Cy7-conjugated anti-B220 (eBioscience), PE-conjugated anti-IgD (BD Pharmingen) and APC-conjugated anti-IgG1 (BD Pharmingen). DAPI was used for the exclusion of dead cells. Data was acquired on a MACSQuant Analyzer (Miltenyi Biotec) and analyzed using FlowJo 7.6.4 software (TreeStar).
Dominguez P.M., Teater M., Chambwe N., Kormaksson M., Redmond D., Ishii J., Vuong B., Chaudhuri J., Melnick A., Vasanthakumar A., Godley L.A., Papavasiliou F.N., Elemento O, & Shaknovich R. (2015). DNA Methylation Dynamics of Germinal Center B Cells Are Mediated by AID. Cell reports, 12(12), 2086-2098.
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4

Comprehensive Flow Cytometry Analysis of Murine Splenocytes

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Flow cytometry analysis of spleen cell suspensions was performed using the following fluorescent-labeled anti–mouse antibodies: APC-conjugated anti-B220 (BD Biosciences #553092), anti-CD138 (BD Biosciences #558626) and anti-IgM (eBioscience #17–5790-82); PE-conjugated anti-IgD (BD Biosciences #558597), anti-CD23 (eBioscience #5010271) and anti-CD95 (BD Biosciences #554258); PECy7-conjugated anti-CD21 (BioLegend #123420), anti-CD95 (BD Biosciences #557653), anti-GL7 (BD Biosciences # 561530) and anti-CD86 (BD Biosciences #560582); PEVio770-conjugated anti-B220 (Miltenyi Biotec #130–102-308); FITC-conjugated anti-CXCR4 (BD Biosciences #551967) and anti-IgG1 (ebioscience #11–4011-85); Brilliant Violet 421-conjugated anti-CD138 (BioLegend #142507); PerCP-Cy5.5-conjugated anti-GL7 (BioLegend #144610), anti-CD138 (BioLegend #142510) and anti-IgM (BD Biosciences #550881), APC-Cy7-conjugated anti-CD19 (ebioscience #47–0193-82), AlexaFluor647-conjugated anti-BLIMP1 (BD Biosciences #563643). Sytox blue (ThermoFisher Scientific) or DAPI was used for the exclusion of dead cells. Data was acquired on a BD FACSCanto II (BD Biosciences) and analyzed using FlowJo v10.1 software (TreeStar).
Dominguez P.M., Ghamlouch H., Rosikiewicz W., Kumar P., Béguelin W., Fontán L., Rivas M.A., Pawlikowska P., Armand M., Mouly E., Torres-Martin M., Doane A.S., Calvo Fernandez M.T., Durant M., Della-Valle V., Teater M., Cimmino L., Droin N., Tadros S., Motanagh S., Shih A.H., Rubin M.A., Tam W., Aifantis I., Levine R.L., Elemento O., Inghirami G., Green M.R., Figueroa M.E., Bernard O.A., Aoufouchi S., Li S., Shaknovich R, & Melnick A.M. (2018). TET2 deficiency causes germinal center hyperplasia, impairs plasma cell differentiation and promotes B-cell lymphomagenesis. Cancer discovery, 8(12), 1632-1653.
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