Log In Sign up
The largest database of trusted experimental protocols

Ethylenediaminetetraacetic acid disodium salt dihydrate

Manufactured by Merck Group
Visit Supplier
Sourced in United States, Switzerland, Germany

Ethylenediaminetetraacetic acid disodium salt dihydrate is a chemical compound used as a chelating agent in various laboratory applications. It is a white, crystalline powder with the molecular formula Na2C10H14N2O8·2H2O. The compound is highly soluble in water and is commonly used to bind and remove metal ions from solutions.

Automatically generated - may contain errors

Lab products found in correlation

Sodium hydroxide (naoh), by Merck Group (6 mentions) Hydrochloric acid (hcl), by Merck Group (5 mentions) Citric acid, by Merck Group (4 mentions) Methanol, by Merck Group (4 mentions) Triton x 100, by Merck Group (3 mentions) Trizma base, by Merck Group (3 mentions) Ammonium hydroxide, by Merck Group (3 mentions) Iron 2 sulfate heptahydrate, by Merck Group (3 mentions) Potassium nitrate, by Merck Group (2 mentions) Milli q system, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Thiourea, by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Poly l arginine hydrochloride, by Merck Group (2 mentions) Calcium chloride dihydrate, by Merck Group (2 mentions) Nitric acid, by Merck Group (2 mentions) Ethanol, by Merck Group (2 mentions) Sodium metasilicate pentahydrate, by Merck Group (2 mentions) 1 1 3 3 tetramethoxypropane, by Merck Group (1 mentions)

Spelling variants of this product

Ethylenediaminetetraacetic acid (561 citations) Ethylenediaminetetraacetic acid disodium salt (17 citations) Disodium salt dihydrate (5 citations) Ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA) (2 citations) Ethylenediaminetetraacetic (EDTA) acid disodium salt dihydrate (2 citations)

22 protocols using ethylenediaminetetraacetic acid disodium salt dihydrate

1

Cytotoxicity Assay Protocol with Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetic acid, DMSO, ethylenediaminetetraAcetic acid disodium salt dihydrate (Na2EDTA·2H2O), FeSO4, L-glutamine, hydrochloric acid (HCl), mannitol, penicillin-G, polyethylene glycol (PEG), pyruvic acid, silver nitrate, NaN3, sodium chloride, sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), sodium sarcosinate, streptomycin sulfate, sulforhodamine B (SRB), 1,1,3,3-tetramethoxypropane, MTT, thiobarbituric acid (TBA), trichloroAcetic acid (TCA), Triton X-100, trizma-Base, trypsin/EDTA (5%), and zinc nitrate, were purchased from Sigma-Aldrich (USA). Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from GibcoBRL, Gaithersburg, MD.
Arooj S., Nazir S., Nadhman A., Ahmad N., Muhammad B., Ahmad I., Mazhar K, & Abbasi R. (2015). Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells. Beilstein Journal of Nanotechnology, 6, 570-582.
+ Open protocol
+ Expand
2

Analysis of St. John's Wort Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were of analytical
reagent grade. Dried St. John’s wort was obtained from a local
market. Sodium fluoride (NaF) was purchased from La Chema, Czech Republic.
Aluminum nitrate nonahydrate (Al(NO3)3·9H2O) and ethylenediaminetetraacetic acid disodium salt dihydrate
(Na2EDTA) were purchased from Sigma-Aldrich. Methyl salicylate
(99.2% pure, 2-(HO)C6H4CO2CH3) was obtained from JQC (Huayin) Pharmaceutical Co., Ltd.,
and sodium acetate (CH3COONa), acetic acid (CH3COOH), ethanol, iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O), iron(II) chloride tetrahydrate (FeCl2·4H2O), manganese(II) nitrate tetrahydrate
(Mn(NO3)2·4H2O), ammonium chloride
(NH4Cl), potassium nitrate (KNO3), calcium nitrate
tetrahydrate (Ca(NO3)2·4H2O),
magnesium nitrate hexahydrate (Mg(NO3)2·6H2O), sodium nitrate (NaNO3), sodium chloride (NaCl),
sodium bromide (NaBr), sodium sulfate (Na2SO4), and boric acid (H3BO3) were purchased from
Merck. Sodium phosphate monobasic (NaH2PO4)
was obtained from Riedel-de Haen.
Yardımcı B., Üzer A, & Apak R. (2022). Spectrophotometric Fluoride Determination Using St. John’s Wort Extract as a Green Chromogenic Complexant for Al(III). ACS Omega, 7(49), 45432-45442.
+ Open protocol
+ Expand
3

Thorium Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ultrapure water was obtained from a MilliQ purification system from Millipore (Bedford, MA, USA). Acetonitrile (ACN) and methanol (MeOH) were LC-MS grade (Optima®) and obtained from Fisher Chemical (Reinach, Switzerland). Trifluoroacetic acid (TFA, ≥99.0%) was obtained from Biosolve BV (Valkenswaard, the Netherlands). Thorium-232 (Th) pure standard at 1.0 mg mL−1 in 2% HNO3 was purchased from PerkinElmer (Schwerzenbach, Switzerland). Ammonium hydroxide (28.0–30.0%), citric acid (≥99.5%), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA, 99.0–101.0%), formic acid (≥98.0%), MES monohydrate (≥99.5%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, ≥98.0%), N-hydroxysuccinimide (≥99.0%), N,N-dimethylacetamide (DMA, 99.8%), p-aminobenzoic acid (pABA, 99.0%), and succinimide (≥98.0%) were obtained from Sigma-Aldrich (Buchs, Switzerland). The 3,2-HOPO chelator and TOP-succinimide, as well as the ACCs, were provided by Bayer AG (Wuppertal, Germany).
Bouvarel T., Bremeyer N., Gao M., Holkenjans W., Hetzel T., Pell R., D’Atri V, & Guillarme D. (2023). Tackling Issues Observed during the Development of a Liquid Chromatography Method for Small Molecule Quantification in Antibody-Chelator Conjugate. Molecules, 28(6), 2626.
+ Open protocol
+ Expand
4

Fabrication of Serotonin Aptamer Biosensor

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prime quality 4″ Si wafers (P/B, 0.001–0.005 Ω-cm, thickness 500 μm) were purchased from Silicon Valley Microelectronics, Inc. (Santa Clara, CA). Sylgard 184® silicone elastomer kits (lot #0008823745) were purchased from Ellsworth Adhesives (Germantown, WI). Indium(III) nitrate hydrate (99.999%), iron nitrate, thiourea, ammonium hydroxide (30% w/v in H2O), hydrogen peroxide (30% v/v in H2O), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), 3-phosphonopropioninc acid, (3-aminopropyl)triethoxysilane (APTES), trimethoxy(propyl)silane, and 3-maleimidobenzoic acid N-hydroxysuccinimide (MBS) were purchased from Sigma-Aldrich (St. Louis, MO) and used as received. UltraPure™ nuclease-free distilled water was purchased from Thermo Fisher Scientific (Waltham, MA) and used as received. The masters templated for lift-off lithography were commercially available DVD-R recordable 16× speed 4.7 GB blank discs (Memorex).
Water was deionized before use (18.2 MΩ-cm) using a Milli-Q system (Millipore, Billerica, MA). The serotonin aptamer (/5ThioMC6-D/CG ACT GGT AGG CAG ATA GGG GAA GCT GAT TCG ATG CGT GGG TCG), thiolated ssDNA (/5ThioMC6-D/GG TTC TTG GAT ATA G), and complementary ssDNA (CTA TAT CCA AGA ACC) were synthesized by Integrated DNA Technologies, Inc. (Coralville, IA). The Ag/AgCl reference electrodes were purchased from World Precision Instruments, Inc. (Sarasota, FL).
Zhao C., Liu Q., Cheung K.M., Liu W., Yang Q., Xu X., Man T., Weiss P.S., Zhou C, & Andrews A.M. (2020). Narrower Nanoribbon Biosensors Fabricated by Chemical Lift-Off Lithography Show Higher Sensitivity. ACS nano, 15(1), 904-915.
+ Open protocol
+ Expand
5

Preparation of Liposomal Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ethylenediaminetetraacetic acid disodium salt dihydrate, potassium phosphate monobasic, disodium phosphate anhydrous, potassium chloride and sodium chloride were purchase from Sigma-Aldrich (USA). Caelyx® was kindly provided by the Pharmacy of the University Hospital of Coimbra (Portugal).
Gregório A.C., Fonseca N.A., Moura V., Lacerda M., Figueiredo P., Simões S., Dias S, & Moreira J.N. (2016). Inoculated Cell Density as a Determinant Factor of the Growth Dynamics and Metastatic Efficiency of a Breast Cancer Murine Model. PLoS ONE, 11(11), e0165817.
+ Open protocol
+ Expand
6

Characterizing Enzyme Kinetics using Spectrophotometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sucrose, Trizma base, Folin–Ciocalteu phenol reagent, ethyleneglycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), deoxycholic acid, trehalose, adenosine triphosphate (ATP), fluorescein isothiocyanate (FITC) and rabbit muscle pyruvate kinase were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Rabbit muscle lactate dehydrogenase was purchased from Roche (Basel, Switzerland). Phosphoenolpyruvate (PEP) and nicotinamide adenine dinucleotide (reduced) salt (NADH) were purchased from Chem-Impex International Inc. (Wood Dale, IL, USA). All other reagents were of the highest quality commercially available.
Rivera-Morán M.A, & Sampedro J.G. (2023). Isolation of the Sarcoplasmic Reticulum Ca2+-ATPase from Rabbit Fast-Twitch Muscle. Methods and Protocols, 6(5), 102.
+ Open protocol
+ Expand
7

Fly Ash Utilization in Water Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Citric acid (99.5–100.5%) and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-Na2—97%) were purchased from Sigma-Aldrich, MO, USA. Ascorbic acid (99%) and tert-butyl alcohol (tBuOH—99%) were supplied by Thermo Fisher Scientific (Waltham, MA, USA). All of the reagents were of analytical grade and used without any further purification. The fly ash used in this study was collected from MSWI plant in Taiwan. RO water was used to prepare all of the aqueous solutions.
Nguyen T.T., Tsai C.K, & Horng J.J. (2022). Sustainable Recovery of Valuable Nanoporous Materials from High-Chlorine MSWI Fly Ash by Ultrasound with Organic Acids. Molecules, 27(7), 2289.
+ Open protocol
+ Expand
8

Fluorescent Dextran Polyelectrolyte Layers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyelectrolytes polystyrenesulfonate sodium (PSS) and polyallylamine hydrochloride (PAH) with a molecular mass of 70 kDa, fluorescein isothiocyanate–dextran (150 kDa) Sigma (USA), Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) purchased in Sigma (St. Louis, MS, USA), sodium dodecyl sulfonate (SDS), sodium carbonate, calcium chloride from “Reahim”.
Dubrovskii A.V., Kim A.L., Musin E.V, & Tikhonenko S.A. (2022). Destruction of polyelectrolyte microcapsules and release of FITC-dextran from them by the influence of sodium dodecyl sulfonate. Scientific Reports, 12, 4032.
+ Open protocol
+ Expand
9

Multimodal Biomaterial Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Calcium chloride (CaCl2, #223506), sodium carbonate (Na2CO3, #S7795), ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA, #E5134), citric acid (HOC(COOH)(CH2COOH)2, #C0759), cerium (III) chloride heptahydrate (CeCl3·7H2O, #22300), gadolinium(III) nitrate hexahydrate (#451134), dextran sulfate sodium salt (DS, MW ≈ 10 kDa, #D4911), 4-iodophenol (#I10201), and poly-L-arginine hydrochloride (PArg, MW ≈ 70 kDa, #P3892), were purchased from Sigma-Aldrich. All chemicals were used as received. Ultrapure water with a resistance greater than 18.2 MΩ cm−1 was used for all experiments. MTT reagent and Hoechst 33,342 dye were purchased from PanEKO (Moscow, Russia). A live/dead assay kit, LDH assay kit, rhodamine B isothiocyanate, and phalloidin-FITC were purchased from Thermo Fisher Scientific, Cambridge, UK.
Kolmanovich D.D., Chukavin N.N., Savintseva I.V., Mysina E.A., Popova N.R., Baranchikov A.E., Sozarukova M.M., Ivanov V.K, & Popov A.L. (2023). Hybrid Polyelectrolyte Capsules Loaded with Gadolinium-Doped Cerium Oxide Nanoparticles as a Biocompatible MRI Agent for Theranostic Applications. Polymers, 15(18), 3840.
+ Open protocol
+ Expand
10

Cellular Uptake and Membrane Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dextran sulphate sodium salt (DS, molecular weight ≈ 10 kDa, #D4911) and poly-L-arginine hydrochloride (PArg, MW ≈ 70 kDa, #P3892), calcium chloride (CaCl2, #223506), sodium carbonate (Na2CO3, #S7795), ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA 2H2O, #E5134), amantadine-HCl (#A1260), genistein(#G6649), amiloride-HCl(#A7410), NaN3 (#199931), cytochalasin D (#C2618),and Hoechst 33,342 (#14533) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). All chemicals were used as received. Ultrapure water with a resistance greater than 18.2 MΩ cm−1 was used for all experiments. Syto 9 (#S34854) and propidium iodide (#BMS500PI), JC-1 (#T3168) and fluorescein isothiocyanate (FITC)-phalloidin (#F432) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Popova N.R., Popov A.L., Ermakov A.M., Reukov V.V, & Ivanov V.K. (2020). Ceria-Containing Hybrid Multilayered Microcapsules for Enhanced Cellular Internalisation with High Radioprotection Efficiency. Molecules, 25(13), 2957.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!